Document Detail


Determination of motility forces of bovine sperm cells using an "optical funnel".
MedLine Citation:
PMID:  10857614     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An optical funnel, a new technique for the evaluation of the force of a microorganism, was applied to the determination of the motility force of bovine sperm cells. In this approach, sperm cells, suspended in an aqueous solution, are introduced into a flow cell, to which radiation pressure is applied from the direction opposite to a medium flow. The sperm cell, which is moving in a stream, is captured by radiation pressure and forced to move to the position at which the force induced by the laser radiation is equal to the force induced by a medium flow. The sperm cell then escapes by its own power on the way to this equilibrium (entrapping) position. The radiation force increases with decreasing distance from the focal point, and as a result, the force of the sperm cell can be determined by measuring the position where the sperm cell escaped against the laser irradiation field. The motility force of the sperm cell was measured in aqueous solution at different pH values and potassium ion concentrations. It was possible to measure more than 250 sperm cells in 3 h. Thus, the optical funnel has potential for use as a rapid and repetitive means for the determination of the motility force of the sperm cell.
Authors:
T Kaneta; N Mishima; T Imasaka
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical chemistry     Volume:  72     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-08-11     Completed Date:  2000-08-11     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2414-7     Citation Subset:  IM    
Affiliation:
Department of Chemical Systems and Engineering, Graduate School of Engineering, Kyushu University, Fukuoka, Japan.
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MeSH Terms
Descriptor/Qualifier:
Algorithms
Animals
Biophysical Phenomena
Biophysics
Cattle
Extracellular Space / metabolism
Hydrogen-Ion Concentration
Male
Sperm Motility / physiology*
Spermatozoa / cytology,  physiology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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