| Determination of mineral content of active dry yeast used in pharmaceutical formulations. | |
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MedLine Citation:
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PMID: 12008125 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The efficiency of seven common methods of digestion of active dry yeast (ADY), which is used in anticariogenic dental formulations, was evaluated for the analytical determination of Fe, Zn, Ca, Mg, Na and K. Four wet-acid digestion and three dry ashing methods are compared in consideration of their estimated reproducibility and metal concentrations obtained. HNO(3), HNO(3)+HCl, HNO(3)+H(2)SO(4) and HNO(3)+HClO(4) were applied for wet digestion of the samples in medium temperatures, while dry ashing at higher temperature with Mg(NO(3))(2) or SrCl(2) as ashing aid agents, were the alternative methods. The final solutions were subsequently analyzed for Fe, Zn, Ca and Mg by flame atomic absorption spectroscopy (FAAS) and for Na and K by atomic emission spectroscopy (AES). Two multivariate statistical methodologies, Analysis of variance (ANOVA) and the Kruskal-Wallis test were applied for the interpretation of the results. Seven additional statistical tests (least-significant difference, Bonferoni, Duncan multiple range, Student-Newman-Keuls, Tuckey significant difference, Tuckey b and Scheffe) were used and proved useful to estimate which of the decomposition methods are outliers. The ideographic approach enabled the comparison of the methods in terms of complexity and difficulty of their steps. Zn and Mg could be reliably determined by any one of the tested methods, while for the other elements, the most powerful method was found and the obtained recoveries were found (>95%). |
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Authors:
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George A Zachariadis; Efthymia S Raidou; Demetrius G Themelis; John A Stratis |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of pharmaceutical and biomedical analysis Volume: 28 ISSN: 0731-7085 ISO Abbreviation: J Pharm Biomed Anal Publication Date: 2002 May |
Date Detail:
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Created Date: 2002-05-14 Completed Date: 2002-07-22 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8309336 Medline TA: J Pharm Biomed Anal Country: England |
Other Details:
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Languages: eng Pagination: 463-73 Citation Subset: IM |
Affiliation:
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Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University, Thessaloniki 54006, Greece. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Data Interpretation, Statistical Indicators and Reagents Metals / analysis Minerals / analysis* Saccharomyces cerevisiae / chemistry* Spectrophotometry, Atomic |
| Chemical | |
Reg. No./Substance:
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0/Indicators and Reagents; 0/Metals; 0/Minerals |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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