Document Detail

Determination of midodrine in human plasma by high-performance liquid chromatography with fluorescence detection.
MedLine Citation:
PMID:  12608767     Owner:  NLM     Status:  MEDLINE    
A simple and sensitive fluorometric high-performance liquid chromatographic method was developed for the determination of midodrine in human plasma. After liquid-liquid extraction from plasma, the drug and 2-phenylglycinol (internal standard) were convened into the corresponding fluorescent derivatives by reaction with 3,4-dihydro-6,7-dimethoxy-4-methyl-3-oxoquinoxaline-2-carbonyl chloride, a fluorescence derivatization reagent for amines. The derivatives were separated within 30 min on a reversed-phase column using isocratic elution with acetonitrile-methanol-water (10:30:60, v/v) and were detected spectrofluorometrically at 485 nm with excitation at 400 nm. The detection limit for midodrine was 0.3 pmol (76 pg) per mL plasma at a signal-to-noise ratio of 3.
Hideyuki Yoshida; Yuki Ohno; Kenichiro Yoshikuni; Kenichiro Todoroki; Hitoshi Nohta; Masatoshi Yamaguchi
Related Documents :
8833977 - Determination of the hiv protease inhibitor bila 2185 bs in rat plasma by liquid-liquid...
1484097 - High-performance liquid chromatographic method for the determination of tris(hydroxymet...
11419747 - Determination of sulfadoxine in human blood plasma using packed-column supercritical fl...
8431677 - Simultaneous determination of salbutamol and terbutaline at overdose levels in human pl...
16665447 - Effects of chilling and aba on [h]gibberellin a(4) metabolism in somatic embryos of gra...
21328537 - Toward a standardized urine proteome analysis methodology.
Publication Detail:
Type:  Journal Article; Validation Studies    
Journal Detail:
Title:  Analytical sciences : the international journal of the Japan Society for Analytical Chemistry     Volume:  19     ISSN:  0910-6340     ISO Abbreviation:  Anal Sci     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-02-28     Completed Date:  2003-07-28     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8511078     Medline TA:  Anal Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  317-9     Citation Subset:  IM    
Faculty of Pharmaceutical Sciences, Fukuoka University, Nanakuma, Johnan, Fukuoka 814-0180, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Adrenergic alpha-Agonists / administration & dosage,  blood,  isolation & purification
Chromatography, High Pressure Liquid / methods*
Midodrine / administration & dosage,  blood*,  pharmacokinetics
Reference Standards
Sensitivity and Specificity
Reg. No./Substance:
0/Adrenergic alpha-Agonists; 0/Quinoxalines; 104077-15-8/3,4-dihydro-6,7-dimethoxy-4-methyl-3-oxoquinoxaline-2-carbonyl chloride; 42794-76-3/Midodrine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Determination of cobalt and nickel by graphite-furnace atomic absorption spectrometry after coprecip...
Next Document:  "Substituent on benzenesulfonyl group" effect in use of 8-benzenesulfonamidoquinoline deri...