Document Detail

Determination of micronucleus frequency by acridine orange fluorescent staining in peripheral blood reticulocytes of mice treated topically with different lubricant oils and cyclophosphamide.
MedLine Citation:
PMID:  17985309     Owner:  NLM     Status:  MEDLINE    
To ascertain whether used and re-refined lubricant oil absorbed through the skin can produce a genotoxic effect or cytotoxicity in mouse bone marrow cells, we examined the induction of micronucleated erythrocytes of peripheral blood after cutaneous application. Both re-refined and used lubricant oils showed a weak but significant induction of micronucleated polychromatic erythrocytes compared with control, while virgin oil did not show micronucleus induction. Cyclophosphamide (CP) was used not only as positive control but also to compare the sensitivity between intraperitoneal and dermal routes of administration of the test compounds in mice. The efficacy of intraperitoneal injection of CP is well known. On the other hand, dermal exposure is not so common and when CP was diluted in glycerin statistically significant values (P = 0.0036) of micronuclei were also found. Topically applied lubricant oils (virgin, re-refined and used) have the capacity to interfere with mouse bone marrow hematopoiesis evidenced by a statistically significant decrease in the proportion of polychromatic erythrocytes in the peripheral blood. Physical and chemical analysis revealed that used oil is more viscous than other lubricants, suggesting the presence of insoluble compounds, oxidized products and water as well as aromatic hydrocarbons. Used oil differs from other lubricant oils in metal and polyaromatic hydrocarbon content. Re-refined oil revealed a neutral value typical of pure mineral oil. This assay is an important tool to evaluate environmental pollutants that cause genotoxicity and/or cytotoxicity through skin exposure.
C R Oliveira-Martins; C K Grisolia
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-09-30
Journal Detail:
Title:  Genetics and molecular research : GMR     Volume:  6     ISSN:  1676-5680     ISO Abbreviation:  Genet. Mol. Res.     Publication Date:  2007  
Date Detail:
Created Date:  2007-11-06     Completed Date:  2007-12-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101169387     Medline TA:  Genet Mol Res     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  566-74     Citation Subset:  IM    
Superintendência de Fiscalização, Agência Nacional do Petróleo, Brasília, DF, Brasil.
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MeSH Terms
Acridine Orange / pharmacology*
Cyclophosphamide / pharmacology*
Fluorescent Dyes / pharmacology
Micronucleus Tests / methods*
Microscopy, Fluorescence / methods
Protein Synthesis Inhibitors / pharmacology
Reticulocytes / drug effects,  metabolism*
Skin / drug effects,  metabolism
Staining and Labeling
Reg. No./Substance:
0/Fluorescent Dyes; 0/Oils; 0/Protein Synthesis Inhibitors; 50-18-0/Cyclophosphamide; 65-61-2/Acridine Orange

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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