Document Detail


Determination of methamphetamine in human blood using microwave extraction-gas chromatography
MedLine Citation:
PMID:  17970127     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
A method was developed for the determination of methamphetamine (MAM) in human blood using microwave extraction-gas chromatography (GC). To improve the extraction efficiency, experimental parameters on the extraction, including such as extraction solvent and its amount, pH value of blood sample, extraction time and temperature were investigated. Comparing with conventional liquid-liquid extraction method, the microwave extraction showed better efficiency under the optimal conditions. The optimal conditions were as follows: the pH of blood sample at 13, ethyl acetate as extraction solvent, extraction at 30 degrees C for 8 min. The average recovery of MAM with this extraction method was 81.4%, and the relative standard deviation was 6.4%. The limit of detection was 220 microg/L for MAM in the blood. Using this method, MAM need not be derivatized and can be separated from the matrix. The results indicate that the developed method is rapid, accurate and sensitive, and can be used for the determination of MAM in blood samples.
Authors:
Hongfeng Sun; Xuexin Gu; Jifen Wang; Weigui Ni; Wenjun Li; Ying Li
Publication Detail:
Type:  English Abstract; Journal Article    
Journal Detail:
Title:  Se pu = Chinese journal of chromatography / Zhongguo hua xue hui     Volume:  25     ISSN:  1000-8713     ISO Abbreviation:  Se Pu     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-10-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9424804     Medline TA:  Se Pu     Country:  China    
Other Details:
Languages:  chi     Pagination:  590-3     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Capital Normal University, Beijing 100037, China. xiaofeng2191@sina.com
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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