| Determination of metformin and its prodrugs in human and rat blood by hydrophilic interaction liquid chromatography. | |
| | |
MedLine Citation:
|
PMID: 19481899 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Simple and specific hydrophilic interaction liquid chromatography (HILIC) method with ultraviolet (UV) detection was developed for the simultaneous determination of highly water-soluble metformin and its more lipophilic prodrugs in human and rat blood samples. The sample preparation was accomplished by precipitating proteins with acetonitrile, which enabled the direct injection of supernatants to the HPLC. Chromatographic separation was performed on an analytical normal phase silica column using a mixture of 0.01 M ammonium acetate pH 5.0 and acetonitrile (40:60, v/v) as a mobile phase at flow rate of 1 ml/min and at the wavelength of 235 nm. The method was validated in terms of specificity, linearity, accuracy, precision, recovery, and analyte stability. The UV-HILIC method was suitable for detecting both metformin and one of its more lipophilic prodrugs simultaneously in human and rat blood samples. |
| | |
Authors:
|
Kristiina M Huttunen; Jarkko Rautio; Jukka Leppänen; Jouko Vepsäläinen; Pekka Keski-Rahkonen |
Related Documents
:
|
23595799 - Controlling liquid spreading using microfabricated undercut edges. 22483219 - A selective metabolite array for the detection of phosphometabolites. 23625759 - Passive sampling for target and non-target analyses of moderately polar and nonpolar su... 23113649 - Brazil nut oil: quality control via triacylglycerol profiles provided by easy ambient s... 23255469 - Simultaneous quantitation of seven pyrethroid metabolites in human urine by capillary g... 15292289 - Steroid profiling by tandem mass spectrometry improves the positive predictive value of... 22009049 - Reflectometric interference spectroscopy (rifs) as a new tool to measure in the complex... 18043909 - Studies on the analytical performance of a non-covalent coating with n,n-didodecyl-n,n-... 22883159 - Reversal of elution order in a single second dimension by changing the first column nat... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-05-07 |
Journal Detail:
|
Title: Journal of pharmaceutical and biomedical analysis Volume: 50 ISSN: 1873-264X ISO Abbreviation: J Pharm Biomed Anal Publication Date: 2009 Oct |
Date Detail:
|
Created Date: 2009-07-20 Completed Date: 2009-11-10 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8309336 Medline TA: J Pharm Biomed Anal Country: England |
Other Details:
|
Languages: eng Pagination: 469-74 Citation Subset: IM |
Affiliation:
|
Department of Pharmaceutical Chemistry, University of Kuopio, P.O. Box 1627, FI-70211 Kuopio, Finland. Kristiina.Huttunen@uku.fi |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Chromatography, High Pressure Liquid / methods* Humans Hypoglycemic Agents / blood*, chemistry Male Metformin / blood*, chemistry Prodrugs / pharmacokinetics* Rats Rats, Wistar Reproducibility of Results |
| Chemical | |
Reg. No./Substance:
|
0/Hypoglycemic Agents; 0/Prodrugs; 657-24-9/Metformin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Simultaneous determination of chondroitin sulfate sodium, allantoin and pyridoxine hydrochloride in ...
Next Document: Stomach rupture associated with noninvasive ventilation