Document Detail


DETERMINATION OF MASLINIC ACID, A PENTACYCLIC TRITERPENE FROM OLIVES, IN RAT PLASMA BY HPLC.
MedLine Citation:
PMID:  23003682     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Maslinic acid, a pentacyclic triterpene from olives, has been reported to exert beneficial effects on health including anticarcinogenic activity. Despite its importance, little is known about its bioavailability in both humans and animals. A fundamental step for this evaluation consisted in measuring this compound in blood. Therefore, a simple HPLC method with diode array detection has been developed. Maslinic acid contained in plasma was extracted twice using ethyl acetate. After centrifugation, the organic fraction was evaporated to dryness and the residue was reconstituted with methanol-water (75:25, v/v) and analyzed by HPLC. The method was validated by obtaining a linear correlation (r2 = 0.999) and an average recovery of 99%. Precision expressed as coefficient of variation ranged from 1.23 to 9.06%. The oral administration of maslinic acid (50 mg/kg) to rats and its subsequent detection in plasma showed that the method is suitable for absorption, distribution, and metabolism studies.
Authors:
Gloria Lozano-Mena; M Emilia Juan; Andros Garcia-Granados; Joana M Planas
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-25
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Evaluation of sexual function in patients presenting with Behçet's disease with or without depressi...
Next Document:  Mast Cells in the Periodontal Disease of Non-HIV-Infected and HIV-Infected Individuals Undergoing Hi...