Document Detail

Determination of lumefantrine in rat plasma by liquid-liquid extraction using LC-MS/MS with electrospray ionization: assay development, validation and application to a pharmacokinetic study.
MedLine Citation:
PMID:  19285925     Owner:  NLM     Status:  MEDLINE    
A simple, sensitive and rapid method for the analysis of lumefantrine in rat plasma using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was developed. Detection was performed by positive ion electrospray ionization (ESI) in multiple reaction monitoring (MRM) mode. The method included a chromatographic run of 5 min using a C(18) analytical column and the calibration curve was linear over the concentration range of 2-500 ng/mL with a correlation coefficient (r) of 0.996 or better. The intra- and inter-day assay precision ranged from 1.5 to 7.5% and 5.5 to 7.7%, respectively, and intra- and inter-day assay accuracy was between 91.3-109.7% and 97.0-104.7%, respectively. The method was successfully applied for the pharmacokinetic study in rats.
Wahajuddin; Sheelendra Pratap Singh; Girish Kumar Jain
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-03
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  877     ISSN:  1873-376X     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-04-06     Completed Date:  2009-05-08     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1133-9     Citation Subset:  IM    
Pharmacokinetics and Metabolism Division, Central Drug Research Institute, Lucknow 226001, Uttar Pradesh, India.
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MeSH Terms
Antimalarials / blood*,  pharmacokinetics
Chromatography, High Pressure Liquid
Ethanolamines / blood*,  pharmacokinetics
Fluorenes / blood*,  pharmacokinetics
Indicators and Reagents
Phenanthrenes / blood,  pharmacokinetics
Rats, Sprague-Dawley
Reference Standards
Reproducibility of Results
Spectrometry, Mass, Electrospray Ionization
Reg. No./Substance:
0/Antimalarials; 0/Ethanolamines; 0/Fluorenes; 0/Indicators and Reagents; 0/Phenanthrenes; 36167-63-2/halofantrine; 82186-77-4/lumefantrine

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