| Determination of kynurenic acid levels in rat brain microdialysis samples and changes in kynurenic acid levels induced by N-acetylaspartic acid. | |
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MedLine Citation:
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PMID: 19816853 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The levels of kynurenic acid, an endogenous antagonist of alpha(7) nicotinic acetylcholine and N-methyl-D-aspartate receptors, were measured in microdialysis samples obtained from the prefrontal cortices of rats using column-switching high-performance liquid chromatography with fluorescence detection. When the perfusate was constantly infused at a rate of 1.0 mu/min, the in vitro recovery of kynurenic acid through the dialysis membrane was approximately 20.4%, and the precision was within 1.31%. Endogenous kynurenic acid in the microdialysis sample was clearly detected using column-switching high-performance liquid chromatography. As an application study, N-acetyl-L-aspartic acid, an endogenous metabolite and precursor of N-acetyl-L-aspartyl-L-glutamic acid, which is an agonist of metabotropic glutamate receptors, was infused for 120 min through the microdialysis probe. The kynurenic acid level significantly increased during the infusion of N-acetyl-L-aspartic acid, suggesting that kynurenic acid might have some association with N-acetyl-L-aspartic acid in vivo. |
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Authors:
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Masayuki Tomiya; Takeshi Fukushima; Tadahiro Ogaya; Yukiko Sone; Shogo Mitsuhashi; Toshimasa Toyo'oka |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biomedical chromatography : BMC Volume: 24 ISSN: 1099-0801 ISO Abbreviation: Biomed. Chromatogr. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-20 Completed Date: 2010-08-09 Revised Date: 2011-02-08 |
Medline Journal Info:
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Nlm Unique ID: 8610241 Medline TA: Biomed Chromatogr Country: England |
Other Details:
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Languages: eng Pagination: 569-73 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2009 John Wiley & Sons, Ltd. |
Affiliation:
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Division of Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aspartic Acid / analogs & derivatives*, analysis Brain Chemistry* Kynurenic Acid / analysis* Male Microdialysis / methods* Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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492-27-3/Kynurenic Acid; 56-84-8/Aspartic Acid; 997-55-7/N-acetylaspartate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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