Document Detail

Determination of keratin degradation by fungi using keratin azure.
MedLine Citation:
PMID:  15283238     Owner:  NLM     Status:  MEDLINE    
Azure dye-impregnated sheep's wool keratin (keratin azure) was incorporated in a high pH medium and overlaid on a keratin-free basal medium. The release and diffusion of the azure dye into the lower layer indicated production of keratinase. Fifty-eight fungal taxa, including 49 members of the Arthrodermataceae, Gymnoascaceae and Onygenaceae (Order Onygenales), were assessed for keratin degradation using this method. The results were comparable to measures of keratin utilization reported in studies using tests based on the perforation or erosion of human hair in vitro.
J A Scott; W A Untereiner
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Medical mycology     Volume:  42     ISSN:  1369-3786     ISO Abbreviation:  Med. Mycol.     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-07-30     Completed Date:  2004-11-16     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  9815835     Medline TA:  Med Mycol     Country:  England    
Other Details:
Languages:  eng     Pagination:  239-46     Citation Subset:  IM    
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MeSH Terms
Azure Stains*
Culture Media / chemistry
Fungi / metabolism*
Hair / metabolism
Indicators and Reagents
Keratins / metabolism*
Mycology / methods
Onygenales / metabolism
Wool / chemistry
Reg. No./Substance:
0/Azure Stains; 0/Culture Media; 0/Indicators and Reagents; 68238-35-7/Keratins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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