Document Detail


Determination of isonicotinic acid in the presence of isoniazid and acetylisoniazid. Studies on isonicotinic acid formation from isoniazid in isolated rat hepatocytes.
MedLine Citation:
PMID:  8704939     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In comparison with the hepatocytes obtained from intact rats and rats pretreated with phenobarbital or 3-methylchoranthrene, the amount of isonicotinic acid (INA) formed from isoniazid (INH) increased substantially after incubation at 37 degrees C using the pretreated hepatocytes. This suggests an oxidative pathway for INA formation from INH, apart from hydrolysis. In order to explore the exact mechanism of INA formation in the hepatocytes, an HPLC assay for INA in the presence of INH and acetylisoniazid was developed. In this assay, INA was extracted after the preparation of an ion pair with tetra-n-butylammonium hydroxide, and analysed using an ODS column and a mobile phase consisting of 0.067 M potassium dihydrogenphosphate solution-methanol (96:4, v/v). The method is simple, accurate and especially suitable for INA determination after incubation of INH in isolated rat hepatocytes.
Authors:
Y Ono; A Noda; Y Zaima; N Jitsufuchi; S Eto; H Noda
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Journal of chromatography. B, Biomedical applications     Volume:  677     ISSN:  1572-6495     ISO Abbreviation:  J. Chromatogr. B, Biomed. Appl.     Publication Date:  1996 Mar 
Date Detail:
Created Date:  1996-09-12     Completed Date:  1996-09-12     Revised Date:  2007-10-16    
Medline Journal Info:
Nlm Unique ID:  9421796     Medline TA:  J Chromatogr B Biomed Appl     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  339-43     Citation Subset:  IM    
Affiliation:
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromatography, High Pressure Liquid
Isoniazid / analogs & derivatives*,  metabolism*
Isonicotinic Acids / metabolism*
Liver / cytology,  drug effects*,  metabolism
Rats
Sensitivity and Specificity
Spectrophotometry, Ultraviolet
Chemical
Reg. No./Substance:
0/Isonicotinic Acids; 1078-38-2/(N)1-acetylisoniazid; 54-85-3/Isoniazid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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