| Determination of isofezolac in biological fluids by reversed-phase liquid column chromatography. | |
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MedLine Citation:
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PMID: 7391179 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A rapid, sensitive, and specific reversed-phase high-performance liquid chromatography assay was developed for the determination of 1,3,4-triphenylpyrazole-5-acetic acid (isofezolac) in plasma and urine. The assay involves extraction into diethyl ether from plasma buffered at pH 4.4. The organic phase is evaporated and the residue, dissolved in the mobile phase [acetonitrile-water-0.2 M phosphate buffer (pH 3) (65 : 15 : 20)] is chromatographed at a flow-rate of 1.5 ml/min. The drug is detected by its UV absorption (detection limit 100 ng/ml) or its very intense fluorescence (detection limit 10 ng/ml). Absolute analytical recoveries for isofezolac varied from 92.9 to 100.4%. The accuracy is ca. 1%. Each separation requires about 6 min. This method was applied successfully to the determination of isofezolac in humans for pharmacokinetic studies. |
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Authors:
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A Bannier; J L Brazier |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography Volume: 182 ISSN: 0021-9673 ISO Abbreviation: J. Chromatogr. Publication Date: 1980 Jun |
Date Detail:
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Created Date: 1980-09-26 Completed Date: 1980-09-26 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0427043 Medline TA: J Chromatogr Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 369-77 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetonitriles Chromatography, High Pressure Liquid / methods Humans Hydrogen-Ion Concentration Indicators and Reagents Pyrazoles / blood*, urine Solvents |
| Chemical | |
Reg. No./Substance:
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0/Acetonitriles; 0/Indicators and Reagents; 0/Pyrazoles; 0/Solvents; 50270-33-2/isofezolac |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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