Document Detail

Determination of isofezolac in biological fluids by reversed-phase liquid column chromatography.
MedLine Citation:
PMID:  7391179     Owner:  NLM     Status:  MEDLINE    
A rapid, sensitive, and specific reversed-phase high-performance liquid chromatography assay was developed for the determination of 1,3,4-triphenylpyrazole-5-acetic acid (isofezolac) in plasma and urine. The assay involves extraction into diethyl ether from plasma buffered at pH 4.4. The organic phase is evaporated and the residue, dissolved in the mobile phase [acetonitrile-water-0.2 M phosphate buffer (pH 3) (65 : 15 : 20)] is chromatographed at a flow-rate of 1.5 ml/min. The drug is detected by its UV absorption (detection limit 100 ng/ml) or its very intense fluorescence (detection limit 10 ng/ml). Absolute analytical recoveries for isofezolac varied from 92.9 to 100.4%. The accuracy is ca. 1%. Each separation requires about 6 min. This method was applied successfully to the determination of isofezolac in humans for pharmacokinetic studies.
A Bannier; J L Brazier
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography     Volume:  182     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1980 Jun 
Date Detail:
Created Date:  1980-09-26     Completed Date:  1980-09-26     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  369-77     Citation Subset:  IM    
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MeSH Terms
Chromatography, High Pressure Liquid / methods
Hydrogen-Ion Concentration
Indicators and Reagents
Pyrazoles / blood*,  urine
Reg. No./Substance:
0/Acetonitriles; 0/Indicators and Reagents; 0/Pyrazoles; 0/Solvents; 7K6U6T1360/isofezolac

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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