Document Detail


Determination of intermediary metabolites in yeast. Critical examination of the effect of sampling conditions and recommendations for obtaining true levels.
MedLine Citation:
PMID:  12464     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of sampling conditions on the levels of adenine nucleotides, pyridine nucleotides, glycolytic intermediates and related metabolites in yeast has been studied. A systematic examination of the conditions for harvesting has shown that it can be best accomplished by rapid filtration. Delays in the handling for removal of the medium, as is usual in the process of obtaining a number of data reported in the literature, lead to important changes in some of the metabolites examined. It is also shown that when a washing is imperative it can be carried out with a methanol-water mixture (50/50, v/v) cooled at -40 degrees without loss of intracellular concentrations of non-readily diffusible metabolites. On the basis of this experience the outline of a generally applicable procedure is presented.
Authors:
M J Sáez; R Lagunas
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  13     ISSN:  0300-8177     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  1976 Nov 
Date Detail:
Created Date:  1977-02-26     Completed Date:  1977-02-26     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  73-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenine Nucleotides / analysis
Ammonia / analysis
Freezing
Glucosephosphates / analysis
Glutamates / analysis
Ketoglutaric Acids / analysis
Methods
NAD / analysis
NADP / analysis
Pyruvates / analysis
Saccharomyces cerevisiae / analysis,  metabolism*
Time Factors
Chemical
Reg. No./Substance:
0/Adenine Nucleotides; 0/Glucosephosphates; 0/Glutamates; 0/Ketoglutaric Acids; 0/Pyruvates; 53-59-8/NADP; 53-84-9/NAD; 7664-41-7/Ammonia

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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