Document Detail


Determination of the in vivo effects of cladribine alone and its combination with cyclophosphamide or cyclophosphamide and mitoxantrone on Bax and Bcl-2 protein expression in B-CLL cells.
MedLine Citation:
PMID:  14767525     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study investigated a correlation between expression of Bcl-2 family members, Bax and Bcl-2 (the Bax/Bcl-2 ratio values) in B-CLL cells in vivo and the response of these cells to chemotherapy. Western blot technique combined with videodensitometry was used for Bax and Bcl-2 determination in homogenate, nuclear and postnuclear fractions of mononuclear cells isolated from peripheral blood of B-CLL patients treated with cladribine alone (C), and in combination with cyclophosphamide (CC) or mitoxantrone and cyclophosphamide (CMC). The Bax/Bcl-2 ratio values were changed in B-CLL cells originated from blood samples of patients treated by the three therapy protocols, and was the most elevated in the case of CMC treatment. High degree of B-CLL cell apoptosis induction with cladribine combined with mitoxantrone and cyclophosphamide was confirmed by DNA fragmentation and an appearance of apoptotic morphology among leukemic cells from the blood of patients treated with this form of combined therapy.
Authors:
Agnieszka Kobylinska; Jerzy Z Blonski; Malgorzata Hanausek; Zbigniew Walaszek; Tadeusz Robak; Zofia M Kilianska
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Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncology reports     Volume:  11     ISSN:  1021-335X     ISO Abbreviation:  Oncol. Rep.     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-02-09     Completed Date:  2004-10-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9422756     Medline TA:  Oncol Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  699-705     Citation Subset:  IM    
Affiliation:
Department of Cytobiochemistry, University of Lodz, 90-237 Lodz, Poland.
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MeSH Terms
Descriptor/Qualifier:
Aged
Aged, 80 and over
Antineoplastic Agents / administration & dosage
Antineoplastic Agents, Alkylating / administration & dosage
Antineoplastic Combined Chemotherapy Protocols / therapeutic use*
Apoptosis
Blotting, Western
Cell Nucleus / metabolism
Cladribine / administration & dosage*
Cyclophosphamide / administration & dosage*,  therapeutic use
DNA Fragmentation
Densitometry
Electrophoresis, Polyacrylamide Gel
Female
Humans
Immunoblotting
Leukemia, B-Cell / drug therapy*
Leukocytes, Mononuclear / metabolism
Male
Middle Aged
Mitoxantrone / administration & dosage*
Proto-Oncogene Proteins / metabolism*
Proto-Oncogene Proteins c-bcl-2 / metabolism*
Subcellular Fractions
bcl-2-Associated X Protein
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Antineoplastic Agents, Alkylating; 0/BAX protein, human; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; 4291-63-8/Cladribine; 50-18-0/Cyclophosphamide; 65271-80-9/Mitoxantrone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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