| Determination of in vivo dose response and allergen-specific T cells in subjects contact-sensitized to squaric acid dibutyl ester. | |
| | |
MedLine Citation:
|
PMID: 18413111 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: Squaric acid dibutyl ester (SADBE) is a known contact sensitizer, but dose-response data are not defined. OBJECTIVE: To determine the relationship between sensitization dose and contact hypersensitivity (CHS) response to SADBE in human volunteers. The study also aimed to investigate whether SADBE-reactive blood T cells could be detected using ex vivo mature dendritic cells (DCs) as antigen-presenting cells. METHOD: Forty healthy volunteers were sensitized to either 12.5, 25, 50, or 250 microg of SADBE in a 48 microL volume. This was followed by elicitation 2 weeks later with five doses (0, 0.2, 2, 20, and 200 microg in 20 microL). An additional 10 subjects received the elicitation doses without prior sensitization. Blood samples obtained after sensitization were purified into T cells and mature DCs. RESULTS: A direct relationship between sensitization dose and in vivo CHS response was observed. The SADBE dose that effectively sensitized 50% of the population (ED50) was 22 microg/cm2. Significant SADBE-specific T-cell proliferation in vitro was not observed 2 weeks after sensitization but became evident after elicitation. CONCLUSION: This study establishes the in vivo dose-response characteristics of immune reactivity to SADBE and antigen-specific T-cell reactivity. |
| | |
Authors:
|
Melissa M Camouse; Alan R Swick; Cindy A Ryan; Ben Hulette; Frank Gerberick; Sally S Tinkle; Susan T Nedorost; Kein D Cooper; Seth R Stevens; Elma D Baron |
Publication Detail:
|
Type: Journal Article; Randomized Controlled Trial |
Journal Detail:
|
Title: Dermatitis : contact, atopic, occupational, drug : official journal of the American Contact Dermatitis Society, North American Contact Dermatitis Group Volume: 19 ISSN: 1710-3568 ISO Abbreviation: - Publication Date: 2008 Mar-Apr |
Date Detail:
|
Created Date: 2008-04-16 Completed Date: 2008-08-07 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101207335 Medline TA: Dermatitis Country: Canada |
Other Details:
|
Languages: eng Pagination: 95-9 Citation Subset: IM |
Affiliation:
|
University Hospitals of Cleveland/Case Medical Center, Cleveland, OH, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adjuvants, Immunologic
/
administration & dosage*,
adverse effects Adolescent Adult Cell Proliferation Coculture Techniques Cyclobutanes / administration & dosage, adverse effects, immunology* Dendritic Cells / immunology Dermatitis, Allergic Contact / etiology, immunology* Dose-Response Relationship, Immunologic Female Humans Immunization Male Middle Aged Skin / immunology* T-Lymphocytes / immunology |
| Chemical | |
Reg. No./Substance:
|
0/Adjuvants, Immunologic; 0/Cyclobutanes; 2892-62-8/squaric acid dibutyl ester |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Efficacy and patient perception of Grenz ray therapy in the treatment of dermatoses refractory to ot...
Next Document: Spa contact dermatitis.