Document Detail


Determination of idebenone in plasma by HPLC/MS.
MedLine Citation:
PMID:  11324455     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To develop a sensitive method for the analysis of idebenone [Ide, 6-(10-hydroxydecyl)-2,3-dimethoxy-5-methyl-p-benzoquinone] in human plasma. METHODS: The concentrations in plasma were determined by a high performance liquid chromatography-mass spectrometry coupled with an atmosphere pressure chemical impact ion source method (LC/MS-APCI). RESULTS: The chromatograms indicated a good separation of the analytes and there was no interference by other compounds. The coefficients of determination of the calibration curves were above 0.999. The calibration range was 20-600 micrograms.L-1. Peak plasma idebenone concentration (Cmax) of (316 +/- 85) micrograms.L-1 was achieved within (96 +/- 34) min (Tpeak) after an oral dose of 30 mg. Lower limit of quantitation of the method for Ide determination was 20 micrograms.L-1. CONCLUSION: The advantages of using LC/MS-APCI technique include better sensitivity, higher selectivity, and less time consumption, compared with HPLC method.
Authors:
P Hu; J Jiang; H H Xie
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta pharmacologica Sinica     Volume:  21     ISSN:  1671-4083     ISO Abbreviation:  Acta Pharmacol. Sin.     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2001-04-27     Completed Date:  2001-06-21     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  100956087     Medline TA:  Acta Pharmacol Sin     Country:  China    
Other Details:
Languages:  eng     Pagination:  306-8     Citation Subset:  IM    
Affiliation:
Clinical Pharmacological Research Center, Peking Union Medical College Hospital, Peking Union Medical College, Beijing 100730, China. peihu@public3.bta.net.cn
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MeSH Terms
Descriptor/Qualifier:
Antioxidants / analysis*,  pharmacology
Benzoquinones / blood*,  pharmacokinetics
Chromatography, High Pressure Liquid / methods
Humans
Mass Spectrometry / methods
Ubiquinone / analogs & derivatives
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Benzoquinones; 1339-63-5/Ubiquinone; 58186-27-9/idebenone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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