| Determination of homovanillic acid (HVA) in human plasma by HPLC with coulometric detection and a new SPE procedure. | |
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MedLine Citation:
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PMID: 16406455 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A sensitive high-performance liquid chromatographic method has been developed for the determination of homovanillic acid (HVA), the main metabolite of dopamine, in human plasma. Analyses were carried out on a reversed-phase column (C8, 250 mm x 4.6 mm i.d., 5 microm) using a mobile phase composed of 10% methanol and 90% aqueous citrate buffer, containing octanesulfonic acid and EDTA at pH 4.8. Coulometric detection was used, setting the guard cell at +0.100 V, the first analytical cell at -0.200 V and the second analytical cell at +0.500 V. A careful solid-phase extraction procedure, based on strong anion exchange (SAX) cartridges (100 mg, 1 mL), was implemented for the pre-treatment of plasma samples. Extraction yield was satisfactory, being the mean value 98.0%. The calibration curve was linear over the concentration range of 0.2-25.0 ng mL(-1) of homovanillic acid. The limit of quantitation (LOQ) was 0.2 ng mL(-1) and the limit of detection (LOD) was 0.1 ng mL(-1). The method was successfully applied to plasma samples from former alcohol, cocaine and heroin addicts. Results were satisfactory in terms of precision and accuracy. Hence, the method is suitable for the determination of homovanillic acid in human plasma. |
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Authors:
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M A Saracino; R Mandrioli; L Mercolini; A Ferranti; A Zaimovic; C Leonardi; M A Raggi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-01-06 |
Journal Detail:
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Title: Journal of pharmaceutical and biomedical analysis Volume: 42 ISSN: 0731-7085 ISO Abbreviation: J Pharm Biomed Anal Publication Date: 2006 Sep |
Date Detail:
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Created Date: 2006-08-28 Completed Date: 2006-11-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8309336 Medline TA: J Pharm Biomed Anal Country: England |
Other Details:
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Languages: eng Pagination: 107-12 Citation Subset: IM |
Affiliation:
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Department of Pharmaceutical Sciences, Faculty of Pharmacy, Alma Mater Studiorum - University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Calibration Chromatography, High Pressure Liquid / methods* Electrochemistry Homovanillic Acid / blood* Humans Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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306-08-1/Homovanillic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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