| Determination of histidine and related compounds in rumen fluid by liquid chromatography. | |
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MedLine Citation:
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PMID: 10692998 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A liquid chromatographic procedure was developed for quantitative determination of histidine (His), histidinol (HDL), histamine (HTM), urocanic acid (URA), imidazolepyruvic acid (ImPA), imidazoleacetic acid (ImAA), and imidazolelactic acid (ImLA) in rumen fluid. The method is based on direct injection analysis by UV absorbance detection at 220 nm. The separation was performed under 2 different chromatographic conditions on a LiChrospher 100 NH2 column. In the first chromatographic system, the mobile phase used for isocratic elution was 67 mM potassium phosphate buffer (monobasic and dibasic) pH 6.45-90% acetonitrile in water (21 + 79); in the second system, an acetonitrile gradient in 63 mM potassium phosphate buffer (monobasic) pH 3.0, obtained by addition of 60 mM phosphoric acid, was used. Analyses of both systems were completed within 32 and 25 min, respectively. The limits of detection of these compounds were (microM): His, 2.8; HDL, 3.7; HTM, 4.0; URA, 0.75; ImPA, 4.7; ImAA, 1.2; and ImLA, 1.3. Recovery of these compounds added to rumen fluid was 97.4-103.0% within a 1-day study and 95.4-99.0% on different day studies. Detectable levels of His were found in the deproteinized rumen fluid of goats, with average concentrations of 16.10, 10.43, 11.14, and 13.62 microM in the rumen fluid collected before the morning feeding and 2, 4, and 6 h after feeding, respectively. HDL, HTM, URA, ImPA, ImAA, and ImLA were not detected in the rumen fluid before and after feeding. Trp, Phe, and Tyr were also identified in the rumen fluid, with average concentrations of 8.25, 29.04, and 12.6 microM, respectively, before the morning feeding. |
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Authors:
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S Wadud; R Onodera; M M Or-Rashid; M R Amin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of AOAC International Volume: 83 ISSN: 1060-3271 ISO Abbreviation: J AOAC Int Publication Date: 2000 Jan-Feb |
Date Detail:
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Created Date: 2000-03-16 Completed Date: 2000-03-16 Revised Date: 2008-03-17 |
Medline Journal Info:
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Nlm Unique ID: 9215446 Medline TA: J AOAC Int Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 8-15 Citation Subset: IM |
Affiliation:
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Miyazaki University, Division of Animal Science, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Body Fluids / chemistry* Chromatography, Liquid / methods* Food Goats Histamine / analysis Histidine / analysis* Histidinol / analysis Hydrogen-Ion Concentration Imidazoles / analysis Lactic Acid / analysis Pyruvates / analysis Rumen / metabolism* Sensitivity and Specificity Urocanic Acid / analysis |
| Chemical | |
Reg. No./Substance:
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0/Imidazoles; 0/Pyruvates; 104-98-3/Urocanic Acid; 30581-89-6/imidazoleacetic acid; 50-21-5/Lactic Acid; 501-28-0/Histidinol; 51-45-6/Histamine; 71-00-1/Histidine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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