Document Detail

Determination of hair cell degeneration and hair cell death in neomycin treated cultures of the neonatal rat cochlea.
MedLine Citation:
PMID:  10082279     Owner:  NLM     Status:  MEDLINE    
The spatial-temporal course of hair cell degeneration and hair cell death was examined in the mammalian cochlea following aminoglycoside treatment. Organotypic cultures were established from postnatal rats (P3) and treated with 1 mM neomycin sulfate for 12-48 h and analyzed using a live/dead assay under epifluorescence microscopy. Live hair cells were labeled with calcein, a probe whose fluorescence and cellular retention depends upon intracellular esterase activity and cell-membrane integrity, respectively. Hair cell death was determined by ethidium homodimer-1, a probe that can enter cells with compromised cell membranes only. Inside the cell it binds to DNA. Hair cell morphology was also examined using phalloidin labeling, scanning electron microscopy and semi-thin section analysis. Results showed that hair cell degeneration and hair cell death occurred in a time dependent gradient from base to apex. After 48 h of neomycin treatment, most apical hair cells survived while most basal hair cells died. Calcein labeling provides a sensitive functional assay for measuring hair cell survival.
H Löwenheim; J Kil; K Gültig; H P Zenner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Hearing research     Volume:  128     ISSN:  0378-5955     ISO Abbreviation:  Hear. Res.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-04-27     Completed Date:  1999-04-27     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7900445     Medline TA:  Hear Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  16-26     Citation Subset:  IM    
Department of Otorhinolaryngology, University of Tübingen, Germany.
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MeSH Terms
Animals, Newborn / physiology
Anti-Bacterial Agents / pharmacology*
Cell Survival / drug effects
Cells, Cultured
Cochlea / innervation*
Hair Cells, Auditory / drug effects*,  physiology,  ultrastructure
Microscopy, Electron, Scanning
Neomycin / pharmacology*
Nerve Degeneration* / pathology
Rats, Sprague-Dawley
Reg. No./Substance:
0/Anti-Bacterial Agents; 1404-04-2/Neomycin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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