Document Detail


Determination of hair cell degeneration and hair cell death in neomycin treated cultures of the neonatal rat cochlea.
MedLine Citation:
PMID:  10082279     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The spatial-temporal course of hair cell degeneration and hair cell death was examined in the mammalian cochlea following aminoglycoside treatment. Organotypic cultures were established from postnatal rats (P3) and treated with 1 mM neomycin sulfate for 12-48 h and analyzed using a live/dead assay under epifluorescence microscopy. Live hair cells were labeled with calcein, a probe whose fluorescence and cellular retention depends upon intracellular esterase activity and cell-membrane integrity, respectively. Hair cell death was determined by ethidium homodimer-1, a probe that can enter cells with compromised cell membranes only. Inside the cell it binds to DNA. Hair cell morphology was also examined using phalloidin labeling, scanning electron microscopy and semi-thin section analysis. Results showed that hair cell degeneration and hair cell death occurred in a time dependent gradient from base to apex. After 48 h of neomycin treatment, most apical hair cells survived while most basal hair cells died. Calcein labeling provides a sensitive functional assay for measuring hair cell survival.
Authors:
H Löwenheim; J Kil; K Gültig; H P Zenner
Related Documents :
3894369 - Growth-plate-chondrocyte profiles and their orientation.
11603759 - Epidermal growth factor upregulates production of supernumerary hair cells in neonatal ...
6178719 - Immunohistochemical localization of several cytoskeletal proteins in inner ear sensory ...
19397899 - New ectopic vestibular hair cell-like cells induced by math1 gene transfer in postnatal...
10082279 - Determination of hair cell degeneration and hair cell death in neomycin treated culture...
8551349 - Regeneration of sensory cells after laser ablation in the lateral line system: hair cel...
11229439 - 4-hydroxynonenal as a biological signal: molecular basis and pathophysiological implica...
22885179 - L-glutamine is a key parameter in the immunosuppression phenomenon.
8390519 - Differential expression and activity of p34cdc2 in cultured aortic adventitial fibrobla...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Hearing research     Volume:  128     ISSN:  0378-5955     ISO Abbreviation:  Hear. Res.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-04-27     Completed Date:  1999-04-27     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7900445     Medline TA:  Hear Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  16-26     Citation Subset:  IM    
Affiliation:
Department of Otorhinolaryngology, University of Tübingen, Germany. hubert.loewenheim@uni-tuebingen.de
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn / physiology
Anti-Bacterial Agents / pharmacology*
Cell Survival / drug effects
Cells, Cultured
Cochlea / innervation*
Hair Cells, Auditory / drug effects*,  physiology,  ultrastructure
Microscopy, Electron, Scanning
Neomycin / pharmacology*
Nerve Degeneration* / pathology
Rats
Rats, Sprague-Dawley
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 1404-04-2/Neomycin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Tympanic membrane displacement patterns in experimental cholesteatoma.
Next Document:  Cisplatin ototoxicity and the possibly protective effect of alpha-melanocyte stimulating hormone.