Document Detail

Determination of growth inhibitory action point of interferon gamma on WISH cells in cell cycle progression and the window of responsiveness of the cells to the interferon.
MedLine Citation:
PMID:  17449270     Owner:  NLM     Status:  MEDLINE    
We had earlier shown that human foetal epithelial cells (WISH), growth-inhibited by interferon gamma (IFNgamma), were reversibly detained at a point prior to DNA synthesis. In the present study, we determined the window of action of IFNgamma in the G1 phase duration and the exact point of detention of WISH cells in cell cycle progression with respect to the known points of detention by the inhibitors of DNA replication initiation (aphidicolin and carbonyl diphosphonate) and of activation of replication protein A (6-dimethylaminopurine), of which RPA activation being the earlier event compared to DNA replication initiation in cell cycle progression. WISH cells, which were released from IFNgamma-induced arrest, permeabilised and exposed independently to these inhibitors show that IFNgamma detains WISH cells prior to initiation of DNA synthesis. Further, exposure of IFNalpha-synchronized (at G0/G1) or mimosine-synchronized (at G1/S) WISH cells to IFNgamma, which was added at different time points post-release from the synchronizing agent, showed that the cells were promptly responsive to the growth inhibitory action of IFNgamma only during the first 11h in G1 phase. Taken together, these results suggest that IFNgamma inhibits growth of WISH cells by detaining them at a point prior to initiation of DNA synthesis and that the IFN acts within the first 11h in G1 phase of the cell cycle.
Surabhi Vashistha; Supriya Patil; Chirag Joshi; Parthasarathi Ajitkumar
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-04-20
Journal Detail:
Title:  Cytokine     Volume:  37     ISSN:  1043-4666     ISO Abbreviation:  Cytokine     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-05-14     Completed Date:  2007-08-15     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9005353     Medline TA:  Cytokine     Country:  United States    
Other Details:
Languages:  eng     Pagination:  108-18     Citation Subset:  IM    
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India.
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MeSH Terms
Cell Cycle / physiology*
Cell Line
Epithelial Cells / cytology,  physiology*
Interferon-alpha / metabolism
Interferon-gamma / metabolism*
Mimosine / metabolism
Reproducibility of Results
Reg. No./Substance:
0/Interferon-alpha; 500-44-7/Mimosine; 82115-62-6/Interferon-gamma

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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