Document Detail


Determination of glucose oxidase immobilised as monolayer onto a flat surface.
MedLine Citation:
PMID:  12088886     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An assay to estimate the amount of glucose oxidase immobilised as a monolayer onto a flat surface is reported. This method is based on the electrochemical detection of the flavin adenine dinucleotide (FAD) cofactor released by the immobilised enzyme in acid solutions. FAD concentration in the acid solution was measured by amperometry, using a flow injection analysis (FIA) system equipped with a wall-jet electrode, and with a sensitivity of (9.2+/-2.0)x10(-2) nA/nM. By this method, the amount of glucose oxidase molecules present in a monolayer deposited on a silanised glass slide was easily detected, in which the detection limit is more than one order of magnitude lower than the maximum loading of the surface with an ordered monolayer of glucose oxidase.
Authors:
Fabio Vianello; Silvia Bortoluzzi; Lucio Zennaro; Adelio Rigo
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biochemical and biophysical methods     Volume:  51     ISSN:  0165-022X     ISO Abbreviation:  J. Biochem. Biophys. Methods     Publication Date:  2002 May 
Date Detail:
Created Date:  2002-06-28     Completed Date:  2002-09-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7907378     Medline TA:  J Biochem Biophys Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  263-71     Citation Subset:  IM    
Affiliation:
Department of Biological Chemistry, University of Padova, via G. Colombo 3, 35121, Padua, Italy.
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MeSH Terms
Descriptor/Qualifier:
Aspergillus niger / enzymology
Electrochemistry / instrumentation,  methods
Enzymes, Immobilized / analysis*
Flavin-Adenine Dinucleotide / analysis
Glass
Glucose Oxidase / analysis*
Microscopy, Atomic Force / methods
Sensitivity and Specificity
Surface Properties
Chemical
Reg. No./Substance:
0/Enzymes, Immobilized; 146-14-5/Flavin-Adenine Dinucleotide; EC 1.1.3.4/Glucose Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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