| Determination of glucose oxidase immobilised as monolayer onto a flat surface. | |
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MedLine Citation:
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PMID: 12088886 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An assay to estimate the amount of glucose oxidase immobilised as a monolayer onto a flat surface is reported. This method is based on the electrochemical detection of the flavin adenine dinucleotide (FAD) cofactor released by the immobilised enzyme in acid solutions. FAD concentration in the acid solution was measured by amperometry, using a flow injection analysis (FIA) system equipped with a wall-jet electrode, and with a sensitivity of (9.2+/-2.0)x10(-2) nA/nM. By this method, the amount of glucose oxidase molecules present in a monolayer deposited on a silanised glass slide was easily detected, in which the detection limit is more than one order of magnitude lower than the maximum loading of the surface with an ordered monolayer of glucose oxidase. |
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Authors:
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Fabio Vianello; Silvia Bortoluzzi; Lucio Zennaro; Adelio Rigo |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of biochemical and biophysical methods Volume: 51 ISSN: 0165-022X ISO Abbreviation: J. Biochem. Biophys. Methods Publication Date: 2002 May |
Date Detail:
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Created Date: 2002-06-28 Completed Date: 2002-09-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7907378 Medline TA: J Biochem Biophys Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 263-71 Citation Subset: IM |
Affiliation:
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Department of Biological Chemistry, University of Padova, via G. Colombo 3, 35121, Padua, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aspergillus niger
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enzymology Electrochemistry / instrumentation, methods Enzymes, Immobilized / analysis* Flavin-Adenine Dinucleotide / analysis Glass Glucose Oxidase / analysis* Microscopy, Atomic Force / methods Sensitivity and Specificity Surface Properties |
| Chemical | |
Reg. No./Substance:
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0/Enzymes, Immobilized; 146-14-5/Flavin-Adenine Dinucleotide; EC 1.1.3.4/Glucose Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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