| Determination of gentamicin in pharmaceutical formulations using peroxyoxalate chemiluminescent detection in flow-injection analysis. | |
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MedLine Citation:
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PMID: 18970635 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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A simple and sensitive procedure for the determination of gentamicin is presented, based on the use of the peroxyoxalate chemiluminescent (PO-CL) system in presence of imidazole as a catalyst. The gentamicin has to be previously derivatized with o-phthaladehyde (OPA) in order to obtain a fluorophore, which participates in the PO reaction, producing a CL emission proportional to the gentamicin concentration. The method is developed by using a particular flow-injection analysis (FIA) manifold, employing sodium dodecyl sulfate (SDS) micellar medium as a carrier in order to avoid the degradation of PO in water. The optimization of the instrumental and chemical variables affecting the CL reaction was rigorously carried out by using experimental design methodology. The method has been successfully applied to pharmaceutical formulations. |
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Authors:
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José M Fernández-Ramos; Ana M García-Campaña; Fermín Alés-Barrero; Juan M Bosque-Sendra |
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Publication Detail:
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Type: Journal Article Date: 2005-12-13 |
Journal Detail:
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Title: Talanta Volume: 69 ISSN: 1873-3573 ISO Abbreviation: Talanta Publication Date: 2006 May |
Date Detail:
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Created Date: 2008-10-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2984816R Medline TA: Talanta Country: England |
Other Details:
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Languages: eng Pagination: 763-8 Citation Subset: - |
Affiliation:
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Department of Analytical Chemistry, Faculty of Sciences, University of Granada, E-18071 Granada, Spain. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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