Document Detail


Determination of gamma-aminobutyric acid by liquid chromatography with electrochemical detection.
MedLine Citation:
PMID:  7061649     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
gamma-Aminobutyric acid (GABA) has been determined in rat brain by derivatization with 2,4,6-trinitrobenzenesulfonic acid. The derivative and an internal standard, 2,4,6-trinitrophenyl-delta-aminovaleric acid, are extracted into toluene and separated by reversed-phase chromatography. Electrochemical reduction of these derivatives permits picomole measurements of GABA in microgram amounts of brain tissue.
Authors:
W L Caudill; G P Houck; R M Wightman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of chromatography     Volume:  227     ISSN:  0021-9673     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1982 Feb 
Date Detail:
Created Date:  1982-05-12     Completed Date:  1982-05-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  331-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain Chemistry*
Chromatography, Liquid / methods
Electrochemistry
Male
Rats
Rats, Inbred Strains
gamma-Aminobutyric Acid / analysis*
Chemical
Reg. No./Substance:
56-12-2/gamma-Aminobutyric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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