Document Detail


Determination of free amino acid enantiomers in rat brain and serum by high-performance liquid chromatography after derivatization with N-tert.-butyloxycarbonyl-L-cysteine and o-phthaldialdehyde.
MedLine Citation:
PMID:  1491056     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The concurrent determination of free amino acid enantiomers and non-chiral amino acids in rat brain and serum was accomplished by high-performance liquid chromatography with fluorimetric detection after derivatization with N-tert.-butyloxycarbonyl-L-cysteine and o-phthaldialdehyde. The method revealed the presence of a large amount of free D-serine (0.22 mumol/g of tissue; D/D + L ratio = 0.25) in the brain whereas D-aspartate and D-alanine were established to be at trace levels. These results further support the presence of D-serine in adult brain tissues as demonstrated by recent work using gas chromatography.
Authors:
A Hashimoto; T Nishikawa; T Oka; K Takahashi; T Hayashi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography     Volume:  582     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1992 Nov 
Date Detail:
Created Date:  1993-03-02     Completed Date:  1993-03-02     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  41-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis*,  blood*
Animals
Brain Chemistry*
Chromatography, High Pressure Liquid / methods*
Cysteine / analogs & derivatives*
Fluorometry
Male
Rats
Rats, Wistar
Stereoisomerism
o-Phthalaldehyde*
Chemical
Reg. No./Substance:
0/Amino Acids; 20887-95-0/N-tert-butyloxycarbonylcysteine; 643-79-8/o-Phthalaldehyde; K848JZ4886/Cysteine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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