| Determination of flavonol metabolites in plasma and tissues of rats by HPLC-radiocounting and tandem mass spectrometry following oral ingestion of [2-(14)C]quercetin-4'-glucoside. | |
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MedLine Citation:
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PMID: 12405795 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Epidemiological studies suggest that consumption of flavonol-rich diets decreases the risk of developing heart disease and certain cancers. Recent studies have detected flavonol conjugates in blood and urine following various dietary interventions. To assess to what extent flavonols also accumulate in tissues, where they might be expected to exert anti-carcinogenic and anti-atherogenic effects, [2-(14)C]quercetin-4'-glucoside was synthesized and fed to rats. After 60 min, 93.6% of the ingested radioactivity was recovered from the intestine, incorporated into 18 metabolites that had undergone deglycosylation followed by varying degrees of glucuronidation, methylation, and/or sulfation. [(14)C]Quercetin, the aglycon of the radiolabeled substrate, was present in the intestine and in trace amounts in the liver but was not detected in the plasma and kidneys. The original [2-(14)C]quercetin-4'-glucoside was detected exclusively in the intestine, where it accounted for only 26.2% of the radioactivity. The remainder of the recovered radioactivity was located mainly in the plasma, liver, and kidneys as (14)C-labeled metabolites. However, compared to the quantities in the gastrointestinal tract, the levels of metabolites in plasma and body tissues were very low, indicating only limited absorption into the blood stream. The data demonstrate that quercetin-4'-glucoside, which is a major flavonol in onions, undergoes rapid and extensive metabolism in the intestine, and this appears not to be associated to any extent with transport across the gut wall into the blood stream. |
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Authors:
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William Mullen; Brigitte A Graf; Stuart T Caldwell; Richard C Hartley; Garry G Duthie; Christine A Edwards; Michael E J Lean; Alan Crozier |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 50 ISSN: 0021-8561 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2002 Nov |
Date Detail:
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Created Date: 2002-10-30 Completed Date: 2002-12-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 6902-9 Citation Subset: IM |
Affiliation:
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Plant Products and Human Nutrition Group, Graham Kerr Building, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbon Radioisotopes / diagnostic use Chromatography, High Pressure Liquid* Flavonoids / analysis*, blood Flavonols Intestines / chemistry Kidney / chemistry Liver / chemistry Male Mass Spectrometry* Quercetin / analogs & derivatives*, analysis, pharmacokinetics* Rats Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/Carbon Radioisotopes; 0/Flavonoids; 0/Flavonols; 117-39-5/Quercetin; 20229-56-5/spiraeoside |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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