Document Detail

Determination of ethyl glucuronide in human serum by hyphenation of capillary isotachophoresis and zone electrophoresis.
MedLine Citation:
PMID:  18383012     Owner:  NLM     Status:  MEDLINE    
The determination of ethyl glucuronide (EtG), a marker of recent alcohol consumption, in human serum by hyphenation of capillary ITP (CITP) and CZE is reported. For CITP step, 1 x 10(-2) M hydrochloric acid adjusted with epsilon-aminocaproic acid (EACA) to pH 4.4 was used as the leading electrolyte, and 1 x 10(-2) M nicotinic acid with EACA, pH 4.4, was used as the terminating electrolyte (TE). All electrolytes contained 0.2% hydroxypropylcellulose to suppress electroosmosis. In CITP, EtG was separated from fast serum macrocomponents chloride, phosphate, lactate, and acetate. Zones of microcomponents including EtG that migrated between acetate and nicotinate were forwarded to the second capillary filled with a BGE identical with the TE. Conductivity detection was used in the CITP step. Sensitive detection in the CZE step was performed using indirect spectrophotometric detection at 254 nm. The assay is based on a 1:5 dilution of serum with deionized water and has a concentration LOD for EtG in diluted sample of 9.8 x 10(-9) M. The method was used for the determination of EtG in sera of volunteers consuming alcohol.
Michaela Nováková; Ludmila Krivánková
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Validation Studies    
Journal Detail:
Title:  Electrophoresis     Volume:  29     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-04-23     Completed Date:  2008-08-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1694-700     Citation Subset:  IM    
Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic.
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MeSH Terms
Electrophoresis / methods*
Electrophoresis, Capillary / methods*
Glucuronates / blood*
Reg. No./Substance:
0/Glucuronates; 17685-04-0/ethyl glucuronide

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