Document Detail


Determination of ethambutol in plasma by high-performance liquid chromatography after pre-column derivatization.
MedLine Citation:
PMID:  9653979     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new HPLC assay using UV detection (200 nm) was developed to determine ethambutol (EMB) concentrations in plasma. Following extraction (0.1 ml plasma) with chloroform, EMB and octylamine (used as internal standard) were derivatized with phenylethylisocyanate. Quantitation in plasma was achieved at 200 nm. There were no interferences from endogenous compounds. Intra- and inter-day variabilities were lower than 5.2 and 7.6%, respectively. The limit of quantitation of the method was 0.2 microg/ml. In plasma, ethambutol was found to be stable for at least one month when samples were stored at -20 degrees C. This assay was applied to the therapeutic monitoring of EMB concentrations in 19 patients suffering from tuberculosis.
Authors:
P Chenevier; L Massias; D Gueylard; R Farinotti
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography. B, Biomedical sciences and applications     Volume:  708     ISSN:  1387-2273     ISO Abbreviation:  J. Chromatogr. B Biomed. Sci. Appl.     Publication Date:  1998 Apr 
Date Detail:
Created Date:  1998-08-24     Completed Date:  1998-08-24     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9714109     Medline TA:  J Chromatogr B Biomed Sci Appl     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  310-5     Citation Subset:  IM    
Affiliation:
Service de Pharmacie Clinique et des Biomatériaux, Hopital Bichat, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Adult
Antitubercular Agents / blood*,  therapeutic use
Chromatography, High Pressure Liquid / methods*
Drug Monitoring
Ethambutol / blood*
Female
Humans
Male
Middle Aged
Reproducibility of Results
Sensitivity and Specificity
Tuberculosis / blood,  drug therapy
Chemical
Reg. No./Substance:
0/Antitubercular Agents; 74-55-5/Ethambutol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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