| Determination of eperisone in human plasma by liquid chromatography-ESI-tandem mass spectrometry. | |
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MedLine Citation:
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PMID: 17958338 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A sensitive and selective method for the determination of 4'-ethyl-3-methyl-3-piperidinopropiophenone hydrochloride (eperisone hydrochloride) in human plasma was developed and validated. The procedure employed an internal standard and a solvent extraction step followed by chromatography on a Xterra C18 minibore column. Detection was by electrospray ionization tandem mass spectrometry with multiple reaction monitoring. The mass transitions of eperisone and tolperisone (IS) were m/z 260 --> 98 and m/z 246 --> 98, respectively. The method has a limit of detection of 0.1 pg/mL for eperisone based on the three times signal-to-noise value with a linear range from 0.01 to 10.0 ng/mL for the analyte. Extraction recovery was on average 98.6 +/- 7.2% (SD) for eperisone. The Intra- and inter-day assay accuracy ranged from 93 to 114% and precision (RSD) was better than 8.5%. The method was successfully employed to analyze plasma samples and evaluate pharmacokinetics of eperisone in healthy male volunteers. |
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Authors:
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Min Kyo Jeoung; Eun Sook Jeong; Nam Hee Kim; Chang-Soo Kim; Youn-Bok Chung; Yong-Moon Lee; Su-Youn Ahn; Hwang-Eui Cho; Yong Hwa Lee; Jin Tae Hong; Dong-Cheul Moon |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Archives of pharmacal research Volume: 30 ISSN: 0253-6269 ISO Abbreviation: Arch. Pharm. Res. Publication Date: 2007 Sep |
Date Detail:
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Created Date: 2007-10-25 Completed Date: 2007-11-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8000036 Medline TA: Arch Pharm Res Country: Korea (South) |
Other Details:
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Languages: eng Pagination: 1174-8 Citation Subset: IM |
Affiliation:
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College of Pharmacy, CBITRC, Chungbuk National University, Cheongju 361-763, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Area Under Curve Calibration Chromatography, High Pressure Liquid / methods* Humans Male Propiophenones / blood* Spectrometry, Mass, Electrospray Ionization / methods* |
| Chemical | |
Reg. No./Substance:
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0/Propiophenones; 56839-43-1/eperisone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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