Document Detail


Determination of eperisone in human plasma by liquid chromatography-ESI-tandem mass spectrometry.
MedLine Citation:
PMID:  17958338     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A sensitive and selective method for the determination of 4'-ethyl-3-methyl-3-piperidinopropiophenone hydrochloride (eperisone hydrochloride) in human plasma was developed and validated. The procedure employed an internal standard and a solvent extraction step followed by chromatography on a Xterra C18 minibore column. Detection was by electrospray ionization tandem mass spectrometry with multiple reaction monitoring. The mass transitions of eperisone and tolperisone (IS) were m/z 260 --> 98 and m/z 246 --> 98, respectively. The method has a limit of detection of 0.1 pg/mL for eperisone based on the three times signal-to-noise value with a linear range from 0.01 to 10.0 ng/mL for the analyte. Extraction recovery was on average 98.6 +/- 7.2% (SD) for eperisone. The Intra- and inter-day assay accuracy ranged from 93 to 114% and precision (RSD) was better than 8.5%. The method was successfully employed to analyze plasma samples and evaluate pharmacokinetics of eperisone in healthy male volunteers.
Authors:
Min Kyo Jeoung; Eun Sook Jeong; Nam Hee Kim; Chang-Soo Kim; Youn-Bok Chung; Yong-Moon Lee; Su-Youn Ahn; Hwang-Eui Cho; Yong Hwa Lee; Jin Tae Hong; Dong-Cheul Moon
Related Documents :
19557238 - Determination of bezafibrate, methotrexate, cyclophosphamide, orlistat and enalapril in...
15578748 - A simple and sensitive assay for the quantitative analysis of paclitaxel in human and m...
19464238 - A highly sensitive and robust uplc-ms with electrospray ionization method for quantitat...
18295558 - Selective and rapid liquid chromatography/negative-ion electrospray ionization mass spe...
19800908 - Structural characterization of n-linked oligosaccharides of defibrase from agkistrodon ...
7240358 - Measurement of ethanolamine- and serine-containing phospholipids by high-performance li...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of pharmacal research     Volume:  30     ISSN:  0253-6269     ISO Abbreviation:  Arch. Pharm. Res.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-10-25     Completed Date:  2007-11-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8000036     Medline TA:  Arch Pharm Res     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  1174-8     Citation Subset:  IM    
Affiliation:
College of Pharmacy, CBITRC, Chungbuk National University, Cheongju 361-763, Korea.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Area Under Curve
Calibration
Chromatography, High Pressure Liquid / methods*
Humans
Male
Propiophenones / blood*
Spectrometry, Mass, Electrospray Ionization / methods*
Chemical
Reg. No./Substance:
0/Propiophenones; 56839-43-1/eperisone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Transport of a new erectogenic udenafil in Caco-2 cells.
Next Document:  UV and IR absorption cross-sections of HCHO, HCDO, and DCDO.