| Determination of environmentally relevant exposure concentrations of polybrominated diphenyl ethers for in vitro toxicological studies. | |
| | |
MedLine Citation:
|
PMID: 20362048 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Toxicological studies at environmentally relevant concentrations are essential for understanding ecotoxic and health risks of pollutants such as polybrominated diphenyl ethers (PBDEs). However, no information is available on what exposure levels of PBDEs in vitro studies are environmentally relevant. We exposed MCF-7, HepG2, H295R and PC12 cells to BDE-47, and measured BDE-47 concentrations in the cells after exposure. We also used the percentile method to summarize literature data on environmental exposure levels of biotic tissues to PBDEs. The exposure concentration that resulted in a BDE-47 burden in cells close to the 90th percentile of PBDEs levels in tissues was assigned as the upper limit for the environmentally relevant concentration. Exposure to 1nM BDE-47 resulted in PBDEs burdens in MCF-7, HepG2 and H295R cells close to the 90th percentile but PBDEs burdens in PC12 cells were higher than the 90th percentile. In consideration of the high exposure levels in PBDE-polluted areas, we concluded that the highest environmentally relevant exposure concentration of PBDEs in culture media should be approximately 10nM for MCF-7, HepG2 and H295R cells, and<10nM for PC12 cells. These results provide an approximate reference for setting environmentally relevant exposure concentrations of PBDEs for studies in vitro. |
| | |
Authors:
|
Rong-Guo Wei; Ya-Xian Zhao; Peng-Yan Liu; Zhan-Fen Qin; Shi-Shuai Yan; Yan Li; Xiao-Fei Qin; Xi-Juan Xia; Xiao-Bai Xu; Min-Chan Yan |
Related Documents
:
|
7830938 - Nerve growth factor and epidermal growth factor rescue pc12 cells from programmed cell ... 12008018 - Analysis of uptake and release of newly synthesized acetylcholine in pc12 cells overexp... 8396168 - A system for characterizing cellular and molecular events in programmed neuronal cell d... 15098 - Regulation of catecholamine biosynthesis in a transplantable rat pheochromocytoma. 22152028 - In vitro effects of 2-methoxyestradiol-bis-sulphamate on reactive oxygen species and po... 15355748 - B cells: new ways to inhibit their function in rheumatoid arthritis. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-31 |
Journal Detail:
|
Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: 24 ISSN: 1879-3177 ISO Abbreviation: Toxicol In Vitro Publication Date: 2010 Jun |
Date Detail:
|
Created Date: 2010-05-17 Completed Date: 2010-06-17 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: England |
Other Details:
|
Languages: eng Pagination: 1078-85 Citation Subset: IM |
Copyright Information:
|
Copyright 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
|
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Line Environmental Exposure / analysis*, standards Environmental Monitoring Environmental Pollutants / metabolism, standards*, toxicity Flame Retardants / metabolism, standards*, toxicity Halogenated Diphenyl Ethers / metabolism, standards*, toxicity Humans Polybrominated Biphenyls / metabolism, standards*, toxicity Toxicity Tests / standards* |
| Chemical | |
Reg. No./Substance:
|
0/Environmental Pollutants; 0/Flame Retardants; 0/Halogenated Diphenyl Ethers; 0/Polybrominated Biphenyls; 0/tetrabrominated diphenyl ether 47 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Optimization of chemically defined cell culture media Replacing Fetal Bovine Serum in mammalian in v...
Next Document: The assessment of estrogenic or anti-estrogenic activity of chemicals by the human stably transfecte...