Document Detail

Determination of the elemental composition of trace analytes in complex matrices using exact masses of product ions and corresponding neutral losses.
MedLine Citation:
PMID:  17546657     Owner:  NLM     Status:  PubMed-not-MEDLINE    
The emergence of time-of-flight (TOF) and hybrid quadrupole/time-of-flight (Q-TOF) mass spectrometers has offered new possibilities for determining the elemental composition of analytes present at trace levels. The mass accuracy provided by these instruments is currently in the range of 2-5 m m/z units, permitting the determination of the elemental composition of small molecules. The orthogonal information of relative isotopic abundances (RIAs) is used to reduce the number of elemental compositions that are possible, based on consideration of exact masses. Elimination of additional possible compositions has been reported when the analyte is fragmented and its resulting product ions and corresponding neutral losses are carefully analyzed. Published algorithms reduce the number of proposed precursor ions by deleting each precursor candidate which cannot be explained by summing any combination of postulated product ion and corresponding neutral loss elemental composition candidates. An extension of such algorithms is described in this paper. This approach compares not only the precursor ion with the different fragments, but tests the possible descent of any ion from all other recorded ions. This extended algorithm has been tested by processing published data. Algorithms analyzing product ion spectra can be used for real-life data. However, there is a risk that an ion which originates from the mobile phase or from a co-eluting matrix compound can be mathematically correlated to the investigated precursor ion. Such an incorrect correlation can lead to the deletion of a correct elemental composition. This is an important issue if TOF rather than Q-TOF instruments are used. Therefore, ultra-performance liquid chromatography (UPLC) and a peak deconvolution algorithm were used to generate and process TOF chromatograms in order to minimize the number of ions which are not related to the analyte precursor ion. The combined use of chromatographic deconvolution and product ion spectra has been tested and is critically discussed.
Anton Kaufmann
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Rapid communications in mass spectrometry : RCM     Volume:  21     ISSN:  0951-4198     ISO Abbreviation:  Rapid Commun. Mass Spectrom.     Publication Date:  2007  
Date Detail:
Created Date:  2007-06-12     Completed Date:  2007-08-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8802365     Medline TA:  Rapid Commun Mass Spectrom     Country:  England    
Other Details:
Languages:  eng     Pagination:  2003-13     Citation Subset:  -    
Copyright Information:
Copyright (c) 2007 John Wiley & Sons, Ltd.
Official Food Control Authority of the Canton of Zurich (Kantonales Labor Zürich), P.O. Box, CH-8030 Zürich, Switzerland.
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