Document Detail


Determination of dopamine in single rat pheochromocytoma cell by capillary electrophoresis with amperometric detection.
MedLine Citation:
PMID:  12860047     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method is described for the direct identification of dopamine in single cultured rat pheochromocytoma cell by capillary electrophoresis (CE) with amperometric detection. The separation and detection conditions were optimized. The dopamine in single cell analysis was identified based on the migration time of standard dopamine and internal standard (epinephrine). The amount of dopamine in a single cell ranged from 0.29 to 1.28 fmol.
Authors:
Liyao Zhang; Sanfu Qv; Zongli Wang; Jieke Cheng
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  792     ISSN:  1570-0232     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-07-15     Completed Date:  2004-03-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  381-5     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Wuhan University, Wuhan 430072, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Dopamine / analysis*
Electrochemistry / methods*
Electrophoresis, Capillary / methods*
PC12 Cells
Pheochromocytoma / chemistry*
Rats
Reference Standards
Tumor Cells, Cultured

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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