Document Detail

Determination of disaturated lecithin in rhesus monkey amniotic fluid as an index of fetal lung maturity.
MedLine Citation:
PMID:  99039     Owner:  NLM     Status:  MEDLINE    
Although increased concentrations of total lecithin in amniotic fluid allow prenatal assessment of fetal lung maturation, it has become clear that routine use of the L/S index may lead to a substantial number of inaccurate predictions. Since disaturated lecithin (DL) is a more specific marker of pulmonary surfactant than total lecithin, we developed a convenient method for measuring this phospholipid in amniotic fluid, and then evaluated its level in pregnant rhesus monkeys of 120 to 163 days of gestation. The method involves osmic acid destruction of unsaturated lipids, chromatographic isolation of disaturated lecithin, and quantitation by phosphorus assay. It can be performed in approximately 5 hours on 4 ml. of amniotic fluid and yield 67 +/- 3 per cent average recovery of added 14C-dipalmitolyl lecithin. The results of analyzing 36 rhesus amniotic fluid specimens showed the disaturated lecithin and the disaturated lecithin/sphingomyelin ratio (DL/S) increase sharply after 150 days of gestation, consistent with the pattern of lung maturation in this species. We conclude that disaturated lecithin can be readily quantitated in primate amniotic fluid and that its concentration, the DL/S ratio, and percentage of disaturated lecithin are potentially useful indices of fetal lung maturity for the clinical laboratory.
V Curbelo; D B Gail; P M Farrell
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of obstetrics and gynecology     Volume:  131     ISSN:  0002-9378     ISO Abbreviation:  Am. J. Obstet. Gynecol.     Publication Date:  1978 Aug 
Date Detail:
Created Date:  1978-10-18     Completed Date:  1978-10-18     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0370476     Medline TA:  Am J Obstet Gynecol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  764-9     Citation Subset:  AIM; IM    
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MeSH Terms
Amniotic Fluid / analysis*,  metabolism
Chromatography, Gas
Chromatography, Thin Layer
Lung / embryology*
Macaca mulatta
Models, Biological
Phosphatidylcholines / analysis*,  metabolism
Pulmonary Surfactants / analysis
Sphingomyelins / analysis,  metabolism
Reg. No./Substance:
0/Phosphatidylcholines; 0/Pulmonary Surfactants; 0/Sphingomyelins

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