Document Detail


Determination of dioxins (PCDDs/PCDFs) and PCBs in food and feed using the DR CALUX bioassay: results of an international validation study.
MedLine Citation:
PMID:  16019820     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Maximum levels for dioxins in food and feedstuffs have been recently established by the European Commission through two regulations. Dioxin-monitoring programmes of food and feedstuffs will therefore be undertaken by the European Member States to implement these regulations, which would be facilitated by fast and low-cost screening methods. Commission Directives 2002/70/EC and 2002/69/EC describe specific characteristics for such screening methods. In the present study, the performance characteristics of the DR CALUX method from BioDetection Systems were established in a validation study with 14 participants. The study was based on two materials (fish oil and feed), each containing four different levels of dioxins and dioxin-like PCBs around the current limits. The results demonstrate that the test is very promising but that in particular the clean-up procedure was a source of variation and requires further optimization and standardization. In addition the quantification is improved by the use of control samples to correct for background contamination, recovery and differences between the TEF values and REP (relative potency) factors in the test.
Authors:
G Gizzi; L A P Hoogenboom; C Von Holst; M Rose; E Anklam
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Validation Studies    
Journal Detail:
Title:  Food additives and contaminants     Volume:  22     ISSN:  0265-203X     ISO Abbreviation:  Food Addit Contam     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-07-15     Completed Date:  2005-10-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8500474     Medline TA:  Food Addit Contam     Country:  England    
Other Details:
Languages:  eng     Pagination:  472-81     Citation Subset:  IM    
Affiliation:
European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Food Safety and Quality Unit, Geel, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Animal Feed / analysis*
Animals
Benzofurans / analysis
Biological Assay / methods
Cell Line, Tumor
Dioxins / analysis*
European Union
Fish Oils / analysis*
Food Contamination / analysis
Gas Chromatography-Mass Spectrometry / methods
Herbicides / analysis
Polychlorinated Biphenyls / analysis*
Rats
Reproducibility of Results
Soil Pollutants / analysis
Teratogens / analysis
Tetrachlorodibenzodioxin / analogs & derivatives,  analysis
Chemical
Reg. No./Substance:
0/Benzofurans; 0/Dioxins; 0/Fish Oils; 0/Herbicides; 0/Polychlorinated Biphenyls; 0/Soil Pollutants; 0/Teratogens; 0/chlorinated dibenzofurans; 0/polychlorodibenzo-4-dioxin; 1746-01-6/Tetrachlorodibenzodioxin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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