| Determination of dicumarol metabolites in bile of rats. | |
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MedLine Citation:
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PMID: 50434 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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After intravenous administration of dicumarol-14C to rats, the bile excreted over the next 24 hr contained from 32 to 46% of the administered radioactivity. At least three primary metabolites and a small amount of unchanged dicumarol were present in the bile. Over 91% of the primary metabolites was converted to dicumarol and 7-hydroxydicumarol by hydrolysis with beta-glucuronidase. Some primary metabolites were hydrolyzed simply by acidification to pH 3 or by treatment under the acidic conditions utilized in the enzymatic hydrolysis. The three primary metabolites contain carboxylic acid groups, as indicated by their electrophoretic mobility-pH profiles, and some are simple glucuronides of dicumarol and 7-hydroxydicumarol. The possibility that others are derivatives of these compounds in which a coumarin lactone ring is opened cannot be ruled out. When the metabolites released by either acidification or enzymatic hydrolysis were chromatographed in n-butanol-3 M ammonia, artifacts were produced, presumably as a result of decomposition of 7-hydroxydicumarol. The question is raised whether a previously reported metabolite (B055) is an artifact. |
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Authors:
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W D Conway; F H Lee; L Neufeld |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of pharmaceutical sciences Volume: 64 ISSN: 0022-3549 ISO Abbreviation: J Pharm Sci Publication Date: 1975 Jul |
Date Detail:
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Created Date: 1975-11-07 Completed Date: 1975-11-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985195R Medline TA: J Pharm Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1158-62 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bile / analysis*, metabolism Chromatography, Paper Dicumarol / analogs & derivatives, analysis*, metabolism Electrophoresis, Paper Feces / analysis Glucuronidase Hydrolysis Male Radioisotope Dilution Technique Rats |
| Chemical | |
Reg. No./Substance:
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66-76-2/Dicumarol; EC 3.2.1.31/Glucuronidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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