| Determination of dextran sulfate sodium with crystal violet by triple-wavelength overlapping resonance Rayleigh scattering. | |
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MedLine Citation:
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PMID: 18674962 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A triple-wavelength overlapping resonance Rayleigh scattering (TWO-RRS) method was developed to detect dextran sulfate sodium (DSS) with crystal violet (CV). At pH 10.0 Britton Robinson buffer solution medium, the interaction of CV with DSS occurred which greatly enhanced the RRS intensity with the new RRS peaks appearing at 340, 501 and 671 nm and all these three peaks enhanced with the increase of DSS concentration in the range of 0.04-2.5 microg ml(-1) and the detection limit for the three single peaks was 0.024, 0.027, and 0.027 microg ml(-1), respectively, whereas that of the TWO-RRS method was 0.013 microg ml(-1). The TWO-RRS method was found to have much better flexibility and high sensitivity than the single-wavelength method. In this paper, the interaction conditions were optimized. The affecting factors and characteristics of RRS for the interaction of DSS with CV were investigated and a sensitive method for the determination of trace amounts of DSS using the TWO-RRS method was developed. |
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Authors:
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Xiu Li Hao; Nian Bing Li; Hong Qun Luo |
Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2008-07-01 |
Journal Detail:
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Title: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Volume: 71 ISSN: 1386-1425 ISO Abbreviation: Spectrochim Acta A Mol Biomol Spectrosc Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2008-11-21 Completed Date: 2009-03-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9602533 Medline TA: Spectrochim Acta A Mol Biomol Spectrosc Country: England |
Other Details:
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Languages: eng Pagination: 1673-7 Citation Subset: IM |
Affiliation:
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School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acids
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pharmacology Dextran Sulfate / chemistry* Gentian Violet / pharmacology* Hydrogen-Ion Concentration Models, Biological Osmolar Concentration Scattering, Radiation* Sensitivity and Specificity Spectrometry, Fluorescence / instrumentation, methods Spectrum Analysis / instrumentation, methods Temperature |
| Chemical | |
Reg. No./Substance:
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0/Acids; 548-62-9/Gentian Violet; 9042-14-2/Dextran Sulfate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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