Document Detail

Determination of desoxyepothilone B in nude mice plasma by liquid-liquid extraction and reversed-phase high-performance liquid chromatography.
MedLine Citation:
PMID:  16616824     Owner:  NLM     Status:  MEDLINE    
A novel reversed-phase high-performance liquid chromatographic (HPLC) method has been established for the determination of a newly developed anti-cancer agent desoxyepothilone B (dEpo B) in nude mice plasma. The sample preparation involved deproteination of 200 microl of plasma sample first, followed by liquid-liquid extraction of the resultant supernatant with chloroform. The compound taxol was used as the internal standard. Chromatographic separations were carried out on a 250 mm x 4.6 mm Zorbax SB-phenyl column with acetonitrile-0.25% orthophosphoric acid (50/50, v/v) as mobile phase and UV detection at 250 nm. For dEpo B and taxol at the concentration level of 10 microg/ml in nude mice plasma, the absolute extraction recoveries were 85.3 and 87.2%, respectively. The linear quantification range of the method was 0.1-100 microg/ml in nude mice plasma with linear correlation coefficients greater than 0.999. The within-day and between-day relative standard deviations (R.S.D.s) for dEpo B at 0.5, 2.5 and 10 microg/ml levels in nude mice plasma fell in the range of 2.8-4.8 and 1.5-4.6%, and the within-day and between-day recoveries were in the range of 96.5-101.7 and 97.7-101.2%, respectively.
Zhi Wang; Hui Wang; Julie K Rhie; Joseph M Covey; Ping Liang; Shuyi Wang; Chun Wang; Yanxue Hu; Gautam Prasad; Li Nan; Donald L Hill; Ruiwen Zhang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-04-17
Journal Detail:
Title:  Journal of pharmaceutical and biomedical analysis     Volume:  42     ISSN:  0731-7085     ISO Abbreviation:  J Pharm Biomed Anal     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-28     Completed Date:  2007-01-19     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  8309336     Medline TA:  J Pharm Biomed Anal     Country:  England    
Other Details:
Languages:  eng     Pagination:  272-6     Citation Subset:  IM    
Department of Pharmacology and Toxicology, Division of Clinical Pharmacology, and Comprehensive Cancer Center, University of Alabama at Birmingham, 35294-0019, USA.
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MeSH Terms
Antineoplastic Agents / blood*,  pharmacokinetics
Chromatography, High Pressure Liquid / methods*
Epothilones / blood*,  pharmacokinetics
Mice, Nude
Reference Standards
Reproducibility of Results
Sensitivity and Specificity
Grant Support
Reg. No./Substance:
0/Antineoplastic Agents; 0/Epothilones; 0/desoxyepothilone B

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