Document Detail

Determination of d-serine and related neuroactive amino acids in human plasma by high-performance liquid chromatography with fluorimetric detection.
MedLine Citation:
PMID:  16890503     Owner:  NLM     Status:  MEDLINE    
A simple and versatile methodology using high-performance liquid chromatography (HPLC) with fluorimetric detection was developed to simultaneously determine d-serine along with other metabolically related neuroactive amino acids in the glutamatergic system: L-serine, L-glutamate, L-glutamine, and glycine. On-column sensitivity was in the lower picomole range. Of two chiral thiol reagents investigated, amino acid derivatives of o-phthaldialdehyde (OPA) in combination with N-isobutyryl-L-cysteine were found to have consistently higher responses than their corresponding N-tert-butyloxycarbonyl-L-cysteine derivatives. This methodology was applied to the quantitative detection of amino acids in human plasma and lays the foundation for further investigations of the role of neuroactive amino acids in the pathophysiology and treatment of neurological and psychiatric disorders.
Suzanne L Grant; Yanina Shulman; Philip Tibbo; David R Hampson; Glen B Baker
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-08-04
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  844     ISSN:  1570-0232     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-11-27     Completed Date:  2007-01-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  278-82     Citation Subset:  IM    
Neurochemical Research Unit, Department of Psychiatry, 12-127 Clinical Sciences Building, University of Alberta, Edmonton T6G 2G3, Canada. <>
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MeSH Terms
Amino Acids / blood*,  chemistry
Chromatography, High Pressure Liquid / methods*
Cysteine / blood,  chemistry
Fluorometry / methods*
Glutamic Acid / blood,  chemistry
Glutamine / blood,  chemistry
Molecular Structure
Serine / blood*,  chemistry
o-Phthalaldehyde / chemistry
Reg. No./Substance:
0/Amino Acids; 52-90-4/Cysteine; 56-45-1/Serine; 56-85-9/Glutamine; 56-86-0/Glutamic Acid; 643-79-8/o-Phthalaldehyde

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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