Document Detail


Determination of the cross-linking effect of adipic acid dihydrazide on glycoconjugate preparation.
MedLine Citation:
PMID:  11003553     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cross-linking effect of adipic acid dihydrazide (ADH) on polysaccharide derivatization can be evaluated by applying combination of elemental analysis and colorimetric assay. Elemental analysis is used for estimation of total ADH bound to polysaccharide and a colorimetric trinitrobenzene sulfonic acid assay is used to determine the part of ADH not involved in cross-linking. The difference of values expressed as molar ratios (per repeating unit) provides information on the amount of ADH involved in cross-linking the polysaccharides. Carboxymethylated polysaccharides were derivatized with different amounts of ADH to test the procedure. Analytical results showed that excess of ADH in the reaction only slightly decreased the cross-linking. The number of carboxyl groups remained unmodified even at high excess of ADH and high concentration of carbodiimide (EDC) coupling reagent.
Authors:
S Bystrický; E Machová; A Malovíková; G Kogan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Glycoconjugate journal     Volume:  16     ISSN:  0282-0080     ISO Abbreviation:  Glycoconj. J.     Publication Date:  1999 Nov 
Date Detail:
Created Date:  2000-09-26     Completed Date:  2000-10-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8603310     Medline TA:  Glycoconj J     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  691-5     Citation Subset:  IM    
Affiliation:
Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovak Republic. chembyst@savba.sk
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MeSH Terms
Descriptor/Qualifier:
Adipic Acids / chemistry*
Carbodiimides / chemistry
Carbohydrate Sequence
Chromatography, High Pressure Liquid
Cross-Linking Reagents / chemistry*
Glycoconjugates / chemistry*
Molecular Sequence Data
Polysaccharides / chemistry
Chemical
Reg. No./Substance:
0/Adipic Acids; 0/Carbodiimides; 0/Cross-Linking Reagents; 0/Glycoconjugates; 0/Polysaccharides; 1071-93-8/adipic dihydrazide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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