| Determination of constants of substrate primary binding with baker's yeast transketolase by kinetic modelling. | |
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MedLine Citation:
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PMID: 9275280 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A kinetic model of bisubstrate reaction catalyzed by baker's yeast transketolase is proposed. The model considers individual stages of substrates reversible primary binding. The model corresponds to the observed kinetics of product accumulation within a wide range of initial substrate concentrations. Kinetic parameters for the best simulation of the experimental data are defined. The equilibrium constants of the primary binding of both the initial and produced ketose and also the initial aldose were unequivocally determined by varying the initial substrate concentrations. The dissociation constants of the primary enzyme-substrate complex for the initial ketose (xylulose 5-phosphate) and the reaction product (sedoheptulose 7-phosphate) were found to differ by more than by two orders of magnitude. The result is discussed in the context of the hypothesis of flip-flop functioning of the transketolase active sites. |
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Authors:
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V A Selivanov; L E Meshalkina; M V Kovina; G A Kochetov |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemistry. Biokhimii͡a Volume: 62 ISSN: 0006-2979 ISO Abbreviation: Biochemistry Mosc. Publication Date: 1997 Apr |
Date Detail:
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Created Date: 1997-09-29 Completed Date: 1997-09-29 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376536 Medline TA: Biochemistry (Mosc) Country: RUSSIA |
Other Details:
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Languages: eng Pagination: 425-32 Citation Subset: IM |
Affiliation:
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Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Russia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Binding Sites Computer Simulation Kinetics Models, Chemical Pentosephosphates / metabolism Protein Binding Recombinant Proteins / metabolism Saccharomyces cerevisiae / enzymology* Sugar Phosphates / metabolism Transketolase / chemistry, genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Pentosephosphates; 0/Recombinant Proteins; 0/Sugar Phosphates; 2646-35-7/sedoheptulose 7-phosphate; 60802-29-1/xylulose-5-phosphate; EC 2.2.1.1/Transketolase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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