| Determination of conjugated linoleic acid in human plasma by fast gas chromatography. | |
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MedLine Citation:
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PMID: 17532324 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new method for the determination of the main isomers of conjugated linoleic acid (CLA) in human and animal plasma was developed by gas chromatography coupled to flame ionization detection (GC-FID). The new method introduces three main advantages in comparison to the current available methodologies: firstly it does not require previous lipid extraction, secondly the chromatographic separation of CLA isomers was performed on an Rtx-2330 column significantly shorter and thinner than the typical long highly polar capillary columns in use that allows a faster analysis than in current methodologies, and thirdly the amount of sample needed to perform the analyses was substantially lower than the amount used in current routine methodologies. Its application to human plasma and rat plasma showed to be robust and reliable for quick and correct identification of the main CLA isomers in particular, and the total fatty acid profile in general, in routine analysis. |
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Authors:
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I Bondia-Pons; C Moltó-Puigmartí; A I Castellote; M C López-Sabater |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Validation Studies Date: 2007-05-10 |
Journal Detail:
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Title: Journal of chromatography. A Volume: 1157 ISSN: 0021-9673 ISO Abbreviation: J Chromatogr A Publication Date: 2007 Jul |
Date Detail:
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Created Date: 2007-07-02 Completed Date: 2007-11-07 Revised Date: 2009-01-15 |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: Netherlands |
Other Details:
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Languages: eng Pagination: 422-9 Citation Subset: IM |
Affiliation:
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Department of Nutrition and Food Science, Reference Center in Food Technology, Faculty of Pharmacy, University of Barcelona, Avenida Joan XXIII s/n, E-08028 Barcelona, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatography, Gas / methods* Humans Linoleic Acid / blood* Rats |
| Chemical | |
Reg. No./Substance:
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2197-37-7/Linoleic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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