| Determination of the composition of mixed micelles of bile salts by kinetic dialysis. | |
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MedLine Citation:
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PMID: 6748869 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A kinetic dialysis method for determining the critical micelle concentration and the composition of mixed micelles has been adapted to study a binary system of bile acids. Using kinetic dialysis, the critical micelle concentrations of taurocholate, deoxycholate and taurodeoxycholate were determined. Monomer analysis was performed by colorimetric and radioactive tracer techniques on aliquots of the dialysates. Similarly, the composition of mixed micelles made from various molar fractions of deoxycholate and taurocholate containing tritium and 14C tracers were determined by this method. The results suggest that, for these bile acids, mixed micelle composition is largely predicted by the molar composition of the binary system. Kinetic dialysis has proven to be a rapid procedure and to yield results that agree with critical micelle concentration values previously reported. |
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Authors:
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M Lake; D T Organisciak |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Lipids Volume: 19 ISSN: 0024-4201 ISO Abbreviation: Lipids Publication Date: 1984 Jul |
Date Detail:
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Created Date: 1984-09-18 Completed Date: 1984-09-18 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 553-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bile Acids and Salts
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analysis* Colloids* Dialysis Kinetics Micelles* Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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EY-01959/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bile Acids and Salts; 0/Colloids; 0/Micelles |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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