Document Detail

Determination of the complete cDNA sequence of rat type II collagen and evaluation of distinct expression patterns of types IIA and IIB procollagen mRNAs during fracture repair in rats.
MedLine Citation:
PMID:  12898314     Owner:  NLM     Status:  MEDLINE    
Elucidating the molecular mechanisms that underlie fracture healing is crucial to understanding and devising strategies for the management of fractures, especially those associated with a pathological condition such as diabetes or old age. Cartilage formation, and therefore the expression of type II collagen by chondrocytes, is a critical step in frac-ture healing. Two forms of type II collagen, IIA and IIB, are known to be produced by alternative splicing of the Alpha(1) (II) procollagen gene. We have followed the patterns of expression of these two forms of type II collagen to determine the nature of chondrocyte recruitment during fracture healing. First, we sequenced the rat collagen type II cDNA to design the primers. Second, using a competitive quantitative reverse transcription-mediated polymerase chain reaction, we provide evidence that (1) there is a basal level of type IIA collagen expression during the early stages of fracture healing; (2) transient but sharp up-regulation of IIA expression occurs concomitant with chondrogenesis and endochondral ossification; and (3) type IIB collagen is the predominant mRNA variant expressed at virtually all times during fracture repair.
Ken Urabe; Hee Joong Kim; Gobinda Sarkar; James T Bronk; Mark E Bolander
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association     Volume:  8     ISSN:  0949-2658     ISO Abbreviation:  J Orthop Sci     Publication Date:  2003  
Date Detail:
Created Date:  2003-08-04     Completed Date:  2003-12-05     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9604934     Medline TA:  J Orthop Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  585-90     Citation Subset:  IM    
Department of Orthopaedic Surgery, Mayo Clinic, Rochester, MN, USA.
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MeSH Terms
Base Sequence / genetics
Collagen Type II / genetics*,  ultrastructure
DNA, Complementary / genetics*
Disease Models, Animal
Femoral Fractures / genetics,  pathology,  therapy
Fracture Healing / genetics*
Gene Expression / genetics*
Procollagen / genetics*,  ultrastructure
RNA, Messenger / genetics*
Rats, Long-Evans
Reverse Transcriptase Polymerase Chain Reaction
Grant Support
Reg. No./Substance:
0/Collagen Type II; 0/DNA, Complementary; 0/Procollagen; 0/RNA, Messenger

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