| Determination of clomipramine and its hydroxylated and demethylated metabolites in plasma and urine by liquid chromatography with electrochemical detection. | |
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MedLine Citation:
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PMID: 3689496 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A procedure for the determination of clomipramine and its 8-hydroxy, demethyl, 8-hydroxydemethyl and didemethyl metabolites in plasma and urine by high-performance liquid chromatography with electrochemical detection is described. A 1-ml plasma or urine sample is made alkaline with a carbonate buffer (pH 9.8) and extracted with 20% ethyl acetate in n-heptane. After back-extraction into an acid phosphate buffer (pH 2.4), an aliquot is injected into a 5-microns ion-paired reversed-phase column and eluted with a mobile phase containing a phosphate buffer with tetramethylammonium chloride-acetonitrile (57:43). The detection is coulometric with a first cell at +0.40 V, a second at +0.73 V and a guard cell set at 0.75 V for oxidation of the mobile phase. The method provides recoveries in the general range of 80-110% and a day-to-day precision of 3.7-8.8%, depending on the compound. The minimum quantifiable level for all compounds was 0.2 ng/ml with a 20-microliters injection. Steady-state plasma concentration data and urinary levels are reported for 24 depressed patients receiving daily either 75-150 mg orally or 50-75 mg by infusion. |
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Authors:
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O Spreux-Varoquaux; D Morin; C Advenier; M Pays |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chromatography Volume: 416 ISSN: 0021-9673 ISO Abbreviation: J. Chromatogr. Publication Date: 1987 May |
Date Detail:
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Created Date: 1987-09-18 Completed Date: 1987-09-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0427043 Medline TA: J Chromatogr Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 311-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, High Pressure Liquid Clomipramine / analogs & derivatives*, analysis*, blood, urine Drug Stability Electrochemistry Humans |
| Chemical | |
Reg. No./Substance:
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303-48-0/desmethylclomipramine; 303-49-1/Clomipramine; 62724-32-7/didemethylclomipramine; 83385-97-1/8-hydroxyclomipramine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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