Document Detail

Determination of cholic acid and chenodeoxycholic acid pool sizes and fractional turnover rates by means of stable isotope dilution technique, making use of deuterated cholic acid and chenodeoxycholic acid.
MedLine Citation:
PMID:  3044647     Owner:  NLM     Status:  MEDLINE    
A procedure is described for the simultaneous determination of cholic acid and chenodeoxycholic acid pool sizes and fractional turnover rates. After oral administration of known amounts of 11,12-dideuterated chenodeoxycholic acid and 2,2,4,4-tetradeuterated cholic acid, the ratios of chenodeoxycholic acid-D2/chenodeoxycholic acid and cholic acid-D4/cholic acid are measured in consecutive serum samples, after which fractional turnover rates and pool sizes of chenodeoxycholic acid and cholic acid are determined arithmetically. In 7 healthy volunteers pool sizes for chenodeoxycholic acid and cholic acid were 22.9 +/- 7.8 and 24.1 +/- 11.7 mumol/kg, respectively. The corresponding values for the fractional turnover rates were 0.23 +/- 0.10 and 0.29 +/- 0.12/day. After oral administration of the labelled bile acids in capsule, the obtained pool sizes were significantly higher than after administration in a bicarbonate solution. Bile acid kinetics were also performed in a patient suffering from a cholesterol synthesis deficiency and in a patient very likely suffering from a bile acid synthesis deficiency. Furthermore, the kinetics of the intestinal absorption and hepatic clearance of unconjugated bile acids have been investigated in 2 healthy subjects.
B J Koopman; F Kuipers; C M Bijleveld; J C van der Molen; G T Nagel; R J Vonk; B G Wolthers
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  175     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  1988 Jul 
Date Detail:
Created Date:  1988-10-13     Completed Date:  1988-10-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  143-55     Citation Subset:  IM    
Central Laboratory for Clinical Chemistry, University Hospital, Groningen, The Netherlands.
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MeSH Terms
Bicarbonates / administration & dosage
Chenodeoxycholic Acid / administration & dosage,  pharmacokinetics*
Cholic Acid
Cholic Acids / administration & dosage,  pharmacokinetics*
Deuterium / diagnostic use
Gas Chromatography-Mass Spectrometry
Indicator Dilution Techniques
Intestinal Absorption / drug effects
Reg. No./Substance:
0/Bicarbonates; 0/Capsules; 0/Cholic Acids; 0/Solutions; 474-25-9/Chenodeoxycholic Acid; 7782-39-0/Deuterium; 81-25-4/Cholic Acid

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