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Determination of chemotherapeutic agents in fish and shellfish by matrix solid-phase dispersion and liquid chromatography-tandem mass spectrometry.
MedLine Citation:
PMID:  23034871     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Chemicals are widely used in aquaculture and one of the main recipients of these analytes is the aquatic environment. The aim of this work was to develop and validate a simple and sensitive method for the determination of multiclass chemotherapeutic agents in farmed fish and shellfish using matrix solid-phase dispersion and liquid chromatography-tandem mass spectrometry. Residues of azamethiphos, three avermectins, two carbamates, and two benzoylureas were extracted from samples using silica gel as clean-up adsorbent and 0.5% acetic acid in acetonitrile as elution solvent. The extraction conditions were investigated and optimized using an experimental design. Mass spectrometry detection was carried out in positive electrospray ionization mode with multiple-reaction monitoring scan (except for benzoylurea family). Matrix-matched standards were used for the drugs quantification. Good linearity (R(2) ≥ 0.996) was observed in the range of 5-500 μg kg(-1) . Limits of detection were in the range of 1.5-3.7 μg kg(-1) . Recoveries from salmon samples spiked with veterinary drugs were in the range 84.9-118%. Precision was satisfactory since relative standard deviations were lower than 10.6%. The method can be successfully applied for the analysis of fish and shellfish from aquaculture.
Authors:
Antonia M Carro; Diego García-Rodríguez; Paula Gonzalez-Siso; Rosa A Lorenzo
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-4
Journal Detail:
Title:  Journal of separation science     Volume:  -     ISSN:  1615-9314     ISO Abbreviation:  J Sep Sci     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088554     Medline TA:  J Sep Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
Dpto. de Química Analítica, Nutrición y Bromatología, Facultad de Química, Universidad de Santiago de Compostela, Santiago de Compostela, Spain.
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