| Determination of the cephalosporin antibiotic cephradine in human plasma by high-performance liquid chromatography with ultraviolet detection. | |
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MedLine Citation:
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PMID: 10798295 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simple and sensitive high-performance liquid chromatographic method, for the determination of cephradine in human plasma samples has been developed and validated. Cephradine and cephaloridine (internal standard) were extracted from human plasma by perchloric acid protein precipitation followed by centrifugation. Aliquots of the extracts were analysed by reversed-phase high-performance liquid chromatography (HPLC) utilising a polymeric reversed-phase PLRP-S column, followed by ultraviolet detection at 260 nm. The method has a working dynamic range from 0.2 to 30.0 microg/ml from 200 microl human plasma. The precision of the method at 0.2 microg/ml was 4.9% (intra-assay) and negligible (inter-assay) as calculated by one-way analysis of variance and the accuracy of the method at 0.2 microg/ml was -4.1% in terms of percentage bias. This method has been successfully applied to clinical studies including an oral bioequivalence study comparing the pharmacokinetics of 500 mg tablets of Kefdrin with 500 mg tablets of Velosef in healthy human volunteers. |
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Authors:
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V M Johnson; J P Allanson; R C Causon |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography. B, Biomedical sciences and applications Volume: 740 ISSN: 1387-2273 ISO Abbreviation: J. Chromatogr. B Biomed. Sci. Appl. Publication Date: 2000 Mar |
Date Detail:
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Created Date: 2000-06-23 Completed Date: 2000-06-23 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9714109 Medline TA: J Chromatogr B Biomed Sci Appl Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 71-80 Citation Subset: IM |
Affiliation:
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Department of International Bioanalysis and Business Support, Glaxo Wellcome Research and Development, Ware, Hertfordshire, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Calibration Cephalosporins / blood*, pharmacokinetics Cephradine / blood*, pharmacokinetics Chromatography, High Pressure Liquid / methods* Clinical Trials as Topic Drug Stability Humans Quality Control Reproducibility of Results Spectrophotometry, Ultraviolet / methods* |
| Chemical | |
Reg. No./Substance:
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0/Cephalosporins; 38821-53-3/Cephradine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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