| Determination of carbohydrate-deficient transferrin separated by lectin affinity chromatography for detecting chronic alcohol abuse. | |
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MedLine Citation:
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PMID: 10481047 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Carbohydrate-deficient transferrin (CDT) has been established as a valuable biological marker for detecting chronic alcohol abuse. To improve the diagnostic efficiency, we studied new CDT determination procedures involving the use of lectin affinity chromatography with Allomyrina dichotoma agglutinin (allo A) and Trichosanthes japonica agglutinin I (TJA-I) to isolate the CDT isoforms CDT-allo A and CDT-TJA, respectively. These procedures, based on detection of the CDT-allo A and CDT-TJA isoforms in sera, showed high sensitivity (100% and 98%, respectively) and high specificity (93% and 85%, respectively). These results demonstrate that the new procedures involving the use of lectin affinity chromatography are more useful for isolating markers in the CDT test than the conventional charge-based separation method. |
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Authors:
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K Yoshikawa; K Umetsu; H Shinzawa; I Yuasa; K Maruyama; T Ohkura; K Yamashita; T Suzuki |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: FEBS letters Volume: 458 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1999 Sep |
Date Detail:
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Created Date: 1999-10-13 Completed Date: 1999-10-13 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 112-6 Citation Subset: IM |
Affiliation:
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Department of Forensic Medicine, Yamagata University School of Medicine, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alcoholism
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blood*,
diagnosis* Biological Markers / blood Carbohydrate-Deficient Glycoprotein Syndrome / blood, diagnosis, genetics Chromatography, Affinity / methods, standards, statistics & numerical data Glycosylation Humans Lectins / diagnostic use*, standards Phenotype Protein Isoforms / isolation & purification, standards Reference Values Statistics, Nonparametric Transferrin / analogs & derivatives*, isolation & purification, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Allomyrina lectin; 0/Biological Markers; 0/Lectins; 0/Protein Isoforms; 0/carbohydrate-deficient transferrin; 11096-37-0/Transferrin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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