Document Detail

Determination of bradykinin in blood by a sensitive radioimmunoassay.
MedLine Citation:
PMID:  1031495     Owner:  NLM     Status:  MEDLINE    
Antibodies of high avidity (maximum Km 1.3 X 10(10) L/M) were produced in rabbits against bradykinin coupled to ovalbumin with toluene 2,4-disocyanate. Tyrosin8- bradykinin was labelled to a specific activity of 530-700 Ci/mmol with 125I by means of lactoperoxidase. Sensitivity of the radioimmunoassay was 0.01 microgram/l blood. Specificity studies demonstrated the essential role of the C-terminal arginine of bradykinin for binding to antibody. Mean recovery of [3H]bradykinin internal standard after the preparation of 86 blood samples was 39.0%. The major loss occurred during ethanol precipitation. In venous blood collected at random conditions from 32 normal subjects the bradykinin concentration ranged 0.04-0.46 microgram/l and showed no sex difference.
U L Hulthén; T Borge
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Scandinavian journal of clinical and laboratory investigation     Volume:  36     ISSN:  0036-5513     ISO Abbreviation:  Scand. J. Clin. Lab. Invest.     Publication Date:  1976 Dec 
Date Detail:
Created Date:  1978-01-27     Completed Date:  1978-01-27     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0404375     Medline TA:  Scand J Clin Lab Invest     Country:  NORWAY    
Other Details:
Languages:  eng     Pagination:  833-9     Citation Subset:  IM    
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MeSH Terms
Bradykinin / blood*,  immunology
Cross Reactions
Peptides / immunology
Radioimmunoassay / methods*
Reg. No./Substance:
0/Peptides; 58-82-2/Bradykinin

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