Document Detail


Determination of bile acids in pharmaceutical formulations using micellar electrokinetic chromatography.
MedLine Citation:
PMID:  10933529     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A micellar electrokinetic chromatography method (MEKC) has been developed and validated for the determination of bile acids (BA) such as ursodeoxycholic acid (UDCA), dehydrocholic acid (DHCA) and deoxycholic acid (DCA) in pharmaceuticals for quality control purpose. The background electrolyte consisted of 20 mM borate-phosphate buffer containing 50 mM sodium dodecylsulfate (SDS), and acetonitrile as additive. UV detection was set at 185 nm. Selectivity, linearity, range, repeatability, intermediate precision and accuracy showed good results. Comparison of the values obtained by MEKC and HPLC methods were in close agreement.
Authors:
V G Rodríguez; S E Lucangioli; G C Fernández Otero; C N Carducci
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pharmaceutical and biomedical analysis     Volume:  23     ISSN:  0731-7085     ISO Abbreviation:  J Pharm Biomed Anal     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2000-11-27     Completed Date:  2000-12-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8309336     Medline TA:  J Pharm Biomed Anal     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  375-81     Citation Subset:  IM    
Affiliation:
Department of Analytical and Physical Chemistry, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Argentina.
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MeSH Terms
Descriptor/Qualifier:
Bile Acids and Salts / analysis*
Chromatography, Micellar Electrokinetic Capillary / methods*
Pharmaceutical Preparations / chemistry*
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry, Ultraviolet
Chemical
Reg. No./Substance:
0/Bile Acids and Salts; 0/Pharmaceutical Preparations

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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