| Determination of betaxolol and its metabolites in blood and urine by high-performance liquid chromatography with fluorimetric detection. | |
| | |
MedLine Citation:
|
PMID: 2094703 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Analytical methods for the determination of betaxolol and two of its metabolites in blood and urine are described. Betaxolol, alpha-hydroxybetaxolol, and the acid metabolite were extracted, with over 65% efficiency, from biological samples by liquid-liquid extraction methods. Analysis was performed using reversed-phase high-performance liquid chromatography with fluorimetric detection. N,N-Dimethyloctylamine (0.005 M) was used to improve the chromatography of betaxolol and alpha-hydroxybetaxolol, while acetic acid (1%) was used for the acid metabolite. An excitation wavelength of 200 nm was found to produce the best detector response. Linear standard curves were obtained for all three compounds with detection limits (signal-to-noise ratio = 3) varying between 1 and 10 ng/ml. The coefficients of variation of the determination for all three compounds in blood and urine varied between 3.0 and 8.7%. The metabolism of betaxolol was studied in twelve healthy male subjects. The amounts (mean +/- S.D.) of betaxolol, alpha-hydroxybetaxolol and the acid metabolite renally excreted in the first 48 h after intravenous administration of 10 mg of betaxolol hydrochloride are 17.1 +/- 6.2, 0.4 +/- 0.1 and 14.5 +/- 3.7%, respectively, of the administered dose. |
| | |
Authors:
|
Y W Wong; T M Ludden |
Related Documents
:
|
21773 - Urinary excretion pattern of methaqualone metabolites in man. 610053 - Cyclic metabolites of etidocaine in humans. 6823553 - Attogram detection limit for aqueous dye samples by laser-induced fluorescence. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of chromatography Volume: 534 ISSN: 0021-9673 ISO Abbreviation: J. Chromatogr. Publication Date: 1990 Dec |
Date Detail:
|
Created Date: 1991-07-02 Completed Date: 1991-07-02 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 0427043 Medline TA: J Chromatogr Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 161-72 Citation Subset: IM |
Affiliation:
|
Department of Pharmacology, University of Texas Health Science Center, San Antonio 78284. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Betaxolol / analogs & derivatives, blood*, metabolism, urine* Chromatography, High Pressure Liquid / methods* Humans Male Spectrometry, Fluorescence |
| Chemical | |
Reg. No./Substance:
|
63659-18-7/Betaxolol; 87549-35-7/alpha-hydroxybetaxolol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: High-performance liquid chromatography of the antihistamine pyrilamine and its N-oxide using electro...
Next Document: Determination of zonisamide (3-sulphamoylmethyl-1,2-benzisoxazole) in plasma at therapeutic concentr...